Plasma Proteins at the Interface of Dental Implants Modulate Osteoblasts Focal Adhesions Expression and Cytoskeleton Organization

Nanomaterials

Simone Lumetti¹  GuidoM. Macaluso¹  Ludovica Parisi¹  Miriam Cutrera¹  Andrea Toffoli¹  MassimilianoG. Bianchi²  Ovidio Bussolati² 

[1] Centro Universitario di Odontoiatria, Dipartimento di Medicina e Chirurgia, Università di Parma, 43126 Parma, Italy;Dipartimento di Medicina e Chirurgia, Università di Parma, 43126 Parma, Italy;

关键词: adsorption;    focal adhesions;    dental implant;    osseointegration;    osteoblasts;    titanium;   

DOI  :  10.3390/nano9101407

来源: DOAJ

【 摘 要 】

The host-material interface is a crucial relationship dictating the possibility of successful osseointegration in implant dentistry. The aim of the present study was to characterize the effects of plasma proteins pre-adsorption on the adhesion capacity of osteoblasts, which occurs immediately after implant insertion in vivo. After having pre-adsorbed human plasma proteins on a machined and microrough titanium surface, MC3T3-E1 osteoblasts adhesion was evaluated through crystal violet cell adhesion assay, immunofluorescence staining for cytoskeleton, focal adhesions and cell nuclei, and scanning electron microscopy. The pre-adsorbed protein layer markedly affected the adhesion rate of cells, as well as their morphology and the expression of focal contacts. Moreover, protein adsorption to the underlying titanium surface was found to be correlated to surface pre-wetting. Thus, the early adsorption of serum proteins to the interface of dental implants impacts cell adhesion in terms of strength and of focal adhesions expression.

【 授权许可】

Unknown