Adaptive evolution of Moniliophthora PR-1 proteins towards its pathogenic lifestyle

BMC Ecology and Evolution

Juliana L. Costa¹  Antonio Figueira¹  Paulo J. P. L. Teixeira²  Gonçalo A. G. Pereira³  Paula F. V. do Prado³  Gabriel L. Fiorin³  Daniela P. T. Thomazella³  Adrielle A. Vasconcelos³  Juliana José³  Renata M. Baroni³  Paulo M. Tokimatu³  Antonio P. Camargo³  Marcelo F. Carazzolle³ 

[1] Centro de Energia Nuclear Na Agricultura, Universidade de São Paulo;Departamento de Ciências Biológicas, Escola Superior de Agricultura “Luiz de Queiroz” (ESALQ), Universidade de São Paulo;Departamento de Genética, Evolução, Microbiologia e Imunologia, Instituto de Biologia, Universidade Estadual de Campinas;

关键词: Witches’ broom disease;    Phytopathogen;    Gene evolution;    Fungi;    Phylogenetics;    Positive selection;   

DOI  :  10.1186/s12862-021-01818-5

来源: DOAJ

【 摘 要 】

Abstract Background Plant pathogenesis related-1 (PR-1) proteins belong to the CAP superfamily and have been characterized as markers of induced defense against pathogens. Moniliophthora perniciosa and Moniliophthora roreri are hemibiotrophic fungi that respectively cause the witches’ broom disease and frosty pod rot in Theobroma cacao. Interestingly, a large number of plant PR-1-like genes are present in the genomes of both species and many are up-regulated during the biotrophic interaction. In this study, we investigated the evolution of PR-1 proteins from 22 genomes of Moniliophthora isolates and 16 other Agaricales species, performing genomic investigation, phylogenetic reconstruction, positive selection search and gene expression analysis. Results Phylogenetic analysis revealed conserved PR-1 genes (PR-1a, b, d, j), shared by many Agaricales saprotrophic species, that have diversified in new PR-1 genes putatively related to pathogenicity in Moniliophthora (PR-1f, g, h, i), as well as in recent specialization cases within M. perniciosa biotypes (PR-1c, k, l) and M. roreri (PR-1n). PR-1 families in Moniliophthora with higher evolutionary rates exhibit induced expression in the biotrophic interaction and positive selection clues, supporting the hypothesis that these proteins accumulated adaptive changes in response to host–pathogen arms race. Furthermore, although previous work showed that MpPR-1 can detoxify plant antifungal compounds in yeast, we found that in the presence of eugenol M. perniciosa differentially expresses only MpPR-1e, k, d, of which two are not linked to pathogenicity, suggesting that detoxification might not be the main function of most MpPR-1. Conclusions Based on analyses of genomic and expression data, we provided evidence that the evolution of PR-1 in Moniliophthora was adaptive and potentially related to the emergence of the parasitic lifestyle in this genus. Additionally, we also discuss how fungal PR-1 proteins could have adapted from basal conserved functions to possible roles in fungal pathogenesis.

【 授权许可】

Unknown