BMC Microbiology | |
Extracytoplasmic function (ECF) sigma factor σF is involved in |
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关键词: Stress response; ECF sigma factor σF; Chromium; Cadmium; Caulobacter crescentus; | |
DOI : 10.1186/1471-2180-12-210 | |
来源: DOAJ |
【 摘 要 】
Abstract
Background
The α-proteobacterium
Results
In this work, we show that the ECF sigma factor σF is one of the regulatory proteins involved in the control of the transcriptional response to chromium and cadmium. Microarray experiments indicate that σF controls eight genes during chromium stress, most of which were previously described as induced by heavy metals. Surprisingly, σF itself is not strongly auto-regulated under metal stress conditions. Interestingly, σF-dependent genes are not induced in the presence of agents that generate reactive oxygen species. Promoter analyses revealed that a conserved σF-dependent sequence is located upstream of all genes of the σF regulon. In addition, we show that the second gene in the
Conclusion
A possible mechanism for induction of the σF-dependent genes by chromium and cadmium is the inactivation of the putative anti-sigma factor NrsF, leading to the release of σF to bind RNA polymerase core and drive transcription of its regulon.
【 授权许可】
Unknown