Size and concentration effects of microplastics on digestion and immunity of hybrid snakehead in developmental stages

Aquaculture Reports

卷:22

Zhengkun Pan¹  Shaodan Wang²  Jixing Zou³  Guohuan Xu³  Chaonan Zhang³ 

[1] Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou 510642, China;

[2] State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China;

[3] Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China;

关键词: Microplastics;    Hybrid snakehead;    Intestinal morphology;    Enzyme activities;    Gene expression;   

DOI  :  

来源: DOAJ

【 摘 要 】

Microplastics pollution has gained much attention for its harmful impact on aquatic organisms. This study aimed to evaluate the size-effects and concentration-effects of microplastics on the physiology of hybrid snakehead (Channa maculate × Channa argus). Parameters including antioxidant enzyme activities, intestinal morphology, immune-related genes, and oxidative-stress-related genes were tested in two life stages development. The results showed hybrid snakehead larvae (0.057 ± 0.009 g in weight) were more intolerant to high concentrations of small-sized microplastics. Hybrid snakehead juvenile (3.87 ± 1.19 g in weight) had been stimulated the immune response and triggered antioxidant defenses under the exposure stress. There were differential expressions of inflammatory and oxidative-stress-related genes coping with differential sizes and concentrations of microplastics. High concentrations of microplastics (2 mg/L, 20 mg/L) stimulated more severe immune response and shortened intestinal villi. In addition, the damaged intestinal morphology and function did not recover after 48 h of depuration. This work is expected to provide a meaningful perspective for better understanding the size and concentration dependent effects and life-stage specific effects of microplastics in hybrid snakehead.

【 授权许可】

Unknown