BioTechniques | 卷:66 |
A method for rapid analysis of the root hydrotropic response in Arabidopsis thaliana | |
María E Campos1  Gladys I Cassab1  L Noriega-Calixto L1  | |
[1] 1Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, Col. Chamilpa, Cuernavaca, Mor., 62210, México; | |
关键词: Arabidopsis thaliana; hydrotropic curvature; moisture gradient; | |
DOI : 10.2144/btn-2018-0173 | |
来源: DOAJ |
【 摘 要 】
The system for analyzing the hydrotropic curvature with a moisture gradient in wild-type Arabidopsis roots was modified. Optimal conditions were determined for detecting a hydrotropic curvature of 90° just after 4 h of stimulation. This system only requires 15 ml of a solution of K2CO3 with a density of 1.48 g·ml-1 to generate a rapid moisture gradient inside a square Petri dish without decreasing root growth. In this, the root growth rate observed in hydrostimulated wild-type and miz1 mutant, utilized as a negative control, increases sixfold compared with those roots examined using the former method.
【 授权许可】
Unknown