Effects of Single and Consortia Inoculants on the Biodegradation Efficiency of Crude Oil in Seawater

International Journal of Technology

卷:13

Nur Zaida Zahari¹  Gan Poh Yan¹  Sahibin Abd Rahim¹ 

[1] Faculty of Science & Natural Resources, Universiti Malaysia Sabah, UMS Road, 88400 Kota Kinabalu, Sabah, Malaysia;

关键词: biodegradation;    crude oil;    seawater;    single and consortia cultures;   

DOI  :  10.14716/ijtech.v13i2.4898

来源: DOAJ

【 摘 要 】

A bioremediation study wasundertaken to assess the biodegradation efficiency of crude oil in seawaterusing two locally isolated strains namely Candida tropicalis RETL-Cr1 and Pseudomonas aeruginosa BAS-Cr1. The inoculation was carried outusing single strains labelled as T1; Candidatropicalis RETL-Cr1, T2; single strain Pseudomonasaeruginosa BAS-Cr1 and T3; mixture of both cultures respectively. Thebiodegradation capability of each strain was examined in a shake-flask cultureat 30?C, agitated at 200 rpm for 28 days. The growth profile was monitored bymeasuring the optical density (OD600) using spectrophotometry. Thebiodegradation efficiency of crude oil was quantified by comparing the initialand final crude oil concentrations, whereas the degradation of selectedaliphatic hydrocarbons was quantified using gas chromatography-massspectrometry (GC-MS) by comparing the initial and final area in chromatograms.The present finding showed that in 5% (v/v) of crude oil, consortia cultureshad the highest degradation, with 50%, while single cultures of C. tropicalisRETL-Cr1 and P. aeruginosa BAS-Cr1achieved 39% and 27%, respectively. The results of biodegradation showed thatconsortia cultures experienced 1.3-fold higher compared to a single culture of C. tropicalis RETL-Cr1 and 2-fold highercompared to a single culture of P.aeruginosa BAS-Cr1. Based on GC-MS analysis, the aliphatic hydrocarbonswere found degraded through the treatment with the highest degradation recordedin consortia cultures: octadecane (73.93%) >eicosane (73.23%) > nonadecane (70.43) > docosane (67.64%) >heptadecane (66.36%) > heneicosane (65.94%) > tricosane (62.28%). From the results obtained, it can beconcluded that the potency of microbes as excellent hydrocarbon degraders is asfollows: consortia (mixed of two species) > C. tropicalisRETL-Cr1> P. aeruginosa BAS-Cr1.This supports the idea that microbial communities, especially in mixtures, havethe ability to degrade hydrocarbon contaminants more effectively and can beenvironmentally friendly due to their specific ability to metabolizehydrocarbons.

【 授权许可】

Unknown