A Peak of H3T3 Phosphorylation Occurs in Synchrony with Mitosis in Sea Urchin Early Embryos

Cells

卷:9

Virginie Glippa¹  Sandrine Ruchaud¹  Fernando Roch¹  Julia Morales¹  Omid Feizbakhsh¹  Patrick Cormier¹  Florian Pontheaux¹ 

[1] Centre National de la Recherche Scientifique (CNRS), Sorbonne Université, Integrative Biology of Marine Models (LBI2M), Station Biologique de Roscoff, CS 90074, 29680 Roscoff CEDEX, France;

关键词: histone phosphorylation;    Haspin;    mitosis;    cell division;    CDK1;    CHR-6494;   

DOI  :  10.3390/cells9040898

来源: DOAJ

【 摘 要 】

The sea urchin embryo provides a valuable system to analyse the molecular mechanisms orchestrating cell cycle progression and mitosis in a developmental context. However, although it is known that the regulation of histone activity by post-translational modification plays an important role during cell division, the dynamics and the impact of these modifications have not been characterised in detail in a developing embryo. Using different immuno-detection techniques, we show that the levels of Histone 3 phosphorylation at Threonine 3 oscillate in synchrony with mitosis in Sphaerechinus granularis early embryos. We present, in addition, the results of a pharmacological study aimed at analysing the role of this key histone post-translational modification during sea urchin early development.

【 授权许可】

Unknown