Distribution pattern of histone H3 phosphorylation at serine 10 during mitosis and meiosis in Brachiaria species

Journal of genetics

A. S. Freitas³  C. M. P. Paula⁴  F. Souza Sobrinho¹  V. H. Techio2² 

[1] Empresa Brasileira de Pesquisa Agropecuária (Embrapa), Embrapa Gado de Leite (CNPGL), Rua Eugênio do Nascimento, 610, Bairro Dom Bosco, CEP: 36038-330 Juiz de Fora-MG, Brazil$$;Universidade Federal de Lavras (UFLA), Campus Universitário, Caixa Postal 3037, CEP: 372000-000 Lavras-MG, Brazil$$;Biological Sciences, Universidade Federal de Lavras (UFLA), Campus Universitário, Caixa Postal 3037, CEP: 372000-000 Lavras-MG, Brazil$$;Genetics and Plant Breeding, Universidade Federal de Lavras (UFLA), Campus Universitário, Caixa Postal 3037, CEP: 372000-000 Lavras-MG, Brazil$$

关键词: sister chromatid cohesion;    cell division;    epigenetics;    histone phosphorylation;    immunodetection.;   

DOI  :  

学科分类：生物科学（综合）

来源: Indian Academy of Sciences

【 摘 要 】

Histones are the major eukaryotic DNA-binding proteins. Posttranslational modifications on N-terminal tails of histones that form nucleosomes are often associated with distinct biological functions. Some theories suggest that one of these modifications, the phosphorylation of histone H3 at serine 10 (H3S10ph) plays a role in both chromosome condensation and sister chromatid cohesion. Although histones and some of their modifications are highly conserved, studies have shown that role and distribution of H3S10ph may differ between species. We evaluated the pattern of H3 phosphorylation using immunodetection during mitosis and meiosis in both diploid and tetraploid genotypes of Brachiaria species. Results revealed differences in chromosome distribution of H3S10ph when mitosis and meiosis were compared. Whole chromosomes were phosphorylated during meiosis I, whereas phosphorylation was restricted to the pericentromeric region in both meiosis II and mitosis. There was no variation in phosphorylation patterns between Brachiaria species and diploid and tetraploid genotypes. Regarding spatiotemporal coordination in the Brachiaria species evaluated, H3S10ph is related to maintenance of sister chromatid cohesion during cell divisions.

【 授权许可】

Unknown