Untargeted Metabolomics Analysis Revealed Lipometabolic Disorders in Perirenal Adipose Tissue of Rabbits Subject to a High-Fat Diet

Animals

卷:11

Tao Tang¹  Yanhong Li¹  Yuan Ma¹  Siqi Xia¹  Tianfu Lai¹  Jiahao Shao¹  Mingchuan Gan¹  Mauricio A. Elzo²  Songjia Lai³  Jie Wang³  Xianbo Jia³ 

[1] College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China;

[2] Department of Animal Science, University of Florida, Gainesville, FL 32611, USA;

[3] Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China;

关键词: high-fat diet;    lipometabolic;    metabolomics;    perirenal adipose tissue (PAT);    rabbit;   

DOI  :  10.3390/ani11082289

来源: DOAJ

【 摘 要 】

A high-fat diet (HFD) is widely recognized as a significant modifiable risk for insulin resistance, inflammation, Type 2 diabetes, atherosclerosis and other metabolic diseases. However, the biological mechanism responsible for key metabolic disorders in the PAT of rabbits subject to HFD remains unclear. Here, untargeted metabolomics (LC-MS/MS) combined with liquid chromatography (LC) and high-resolution mass spectrometry (MS) were used to evaluate PAT metabolic changes. Histological observations showed that the adipocytes cells and density of PAT were significantly increased in HFD rabbits. Our study revealed 206 differential metabolites (21 up-regulated and 185 down-regulated); 47 differential metabolites (13 up-regulated and 34 down-regulated), comprising mainly phospholipids, fatty acids, steroid hormones and amino acids, were chosen as potential biomarkers to help explain metabolic disorders caused by HFD. These metabolites were mainly associated with the biosynthesis of unsaturated fatty acids, the arachidonic acid metabolic pathway, the ovarian steroidogenesis pathway, and the platelet activation pathway. Our study revealed that a HFD caused significant lipometabolic disorders. These metabolites may inhibit oxygen respiration by increasing the adipocytes cells and density, cause mitochondrial and endoplasmic reticulum dysfunction, produce inflammation, and finally lead to insulin resistance, thus increasing the risk of Type 2 diabetes, atherosclerosis, and other metabolic syndromes.

【 授权许可】

Unknown