Genome-Wide Association Analysis for Candidate Genes Contributing to Kernel-Related Traits in Maize

Frontiers in Plant Science

卷:13

Jiquan Xue¹  Ting Li¹  Zhibo Qu¹  Ying Wu²  Hangyu Liang²  Shutu Xu²  Die Hu³  Fan Ye³ 

[1] F University, Yangling, China;

[2] Key Laboratory of Biology and Genetic Improvement of Maize in Arid Area of Northwest Region, Ministry of Agriculture and Rural Affairs, College of Agronomy, Northwest A&

[3] Maize Engineering Technology Research Centre, Yangling, China;

关键词: genome-wide association study;    expression profile;    kernel-related traits;    maize;    differential expression;   

DOI  :  10.3389/fpls.2022.872292

来源: DOAJ

【 摘 要 】

Maize grain size is the main factor determining grain yield. Dissecting the genetic basis of maize grain size may help reveal the regulatory mechanism of maize seed development and yield formation. In this study, two associated populations were used for genome-wide association analysis of kernel length, kernel width, kernel thickness, and hundred-kernel weight from multiple locations in AM122 and AM180, respectively. Then, genome-wide association mapping was performed based on the maize 6H90K SNP chip. A total of 139 loci were identified as associated with the four traits with p < 1 × 10−4 using two models (FarmCPU and MLM). The transcriptome data showed that 15 of them were expressed differentially in two maize-inbred lines KB182 (small kernel) and KB020 (big kernel) during kernel development. These candidate genes were enriched in regulating peroxidase activity, oxidoreductase, and leaf senescence. The molecular function was major in binding and catalytic activity. This study provided important reference information for exploring maize kernel development mechanisms and applying molecular markers in high-yield breeding.

【 授权许可】

Unknown