A Precisely Flow-Controlled Microfluidic System for Enhanced Pre-Osteoblastic Cell Response for Bone Tissue Engineering

Bioengineering

卷:5

Maria Chatzinikolaidou¹  Eleftheria Babaliari¹  George Petekidis¹ 

[1] Department of Materials Science and Technology, University of Crete, Crete 70013, Greece;

关键词: microfluidics;    collagen;    osteogenic differentiation;    cell orientation;    MC3T3-E1 pre-osteoblasts;   

DOI  :  10.3390/bioengineering5030066

来源: DOAJ

【 摘 要 】

Bone tissue engineering provides advanced solutions to overcome the limitations of currently used therapies for bone reconstruction. Dynamic culturing of cell-biomaterial constructs positively affects the cell proliferation and differentiation. In this study, we present a precisely flow-controlled microfluidic system employed for the investigation of bone-forming cell responses cultured on fibrous collagen matrices by applying two flow rates, 30 and 50 μL/min. We characterized the collagen substrates morphologically by means of scanning electron microscopy, investigated their viscoelastic properties, and evaluated the orientation, proliferation and osteogenic differentiation capacity of pre-osteoblastic cells cultured on them. The cells are oriented along the direction of the flow at both rates, in contrast to a random orientation observed under static culture conditions. The proliferation of cells after 7 days in culture was increased at both flow rates, with the flow rate of 50 μL/min indicating a significant increase compared to the static culture. The alkaline phosphatase activity after 7 days increased at both flow rates, with the rate of 30 μL/min indicating a significant enhancement compared to static conditions. Our results demonstrate that precisely flow-controlled microfluidic cell culture provides tunable control of the cell microenvironment that directs cellular activities involved in bone regeneration.

【 授权许可】

Unknown