Development of Collagen/Demineralized Bone Powder Scaffolds and Periosteum-Derived Cells for Bone Tissue Engineering Application

International Journal of Molecular Sciences

Thakoon Thitiset⁴  Siriporn Damrongsakkul¹  Tanom Bunaprasert³  Wilairat Leeanansaksiri² 

[1] Department of Chemical Engineering, Faculty of Engineering, Chulalongkorn University, Bangkok 10330, Thailand; E-Mail:;Stem Cell Therapy and Transplantation Research Group, Institute of Sciences, School of Microbiology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand; E-Mail:;Division of Facial Plastic and Reconstructive Surgery, Department of Otolaryngology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand; E-Mail:;Interdisciplinary Program in Biomedical Engineering, Faculty of Engineering, Chulalongkorn University, Bangkok 10330, Thailand; E-Mail:

关键词: collagen;    demineralized bone powder;    osteogenic differentiation;    periosteum-derived cells;    scaffold;   

DOI  :  10.3390/ijms14012056

来源: mdpi

【 摘 要 】

The aim of this study was to investigate physical and biological properties of collagen (COL) and demineralized bone powder (DBP) scaffolds for bone tissue engineering. DBP was prepared and divided into three groups, based on various particle sizes: 75–125 μm, 125–250 μm, and 250–500 μm. DBP was homogeneously mixed with type I collagen and three-dimensional scaffolds were constructed, applying chemical crosslinking and lyophilization. Upon culture with human periosteum-derived cells (PD cells), osteogenic differentiation of PD cells was investigated using alkaline phosphatase (ALP) activity and calcium assay kits. The physical properties of the COL/DBP scaffolds were obviously different from COL scaffolds, irrespective of the size of DBP. In addition, PD cells cultured with COL scaffolds showed significantly higher cell adhesion and proliferation than those with COL/DBP scaffolds. In contrast, COL/DBP scaffolds exhibited greater osteoinductive potential than COL scaffolds. The PD cells with COL/DBP scaffolds possessed higher ALP activity than those with COL scaffolds. PD cells cultured with COL/DBP scaffolds with 250–500 μm particle size yielded the maximum calcium deposition. In conclusion, PD cells cultured on the scaffolds could exhibit osteoinductive potential. The composite scaffold of COL/DBP with 250–500 μm particle size could be considered a potential bone tissue engineering implant.

【 授权许可】

CC BY   
© 2013 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.