Development of a quantitative fluorescence-based lateral flow immunoassay for determination of chloramphenicol, thiamphenicol and florfenicol in milk

Food and Agricultural Immunology

卷:29

Xi Xia¹  Sanlei Xie¹  Jianyi Wang¹  Haiyang Jiang¹  Kai Yao¹  Tao Peng¹  Jiancheng Li¹  Qi Wang¹  Xiya Zhang¹  Yongjun Zheng² 

[1] Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University;

[2] Department of Mechanical and Electrical Engineering, College of Engineering, China Agricultural University;

关键词: lateral flow immunoassay;    quantitative detection;    fluorescent microsphere;    chloramphenicol;    thiamphenicol;    florfenicol;    milk;   

DOI  :  10.1080/09540105.2017.1359498

来源: DOAJ

【 摘 要 】

A competitive lateral flow immunoassay (LFA) using fluorescent microspheres (FMs) as label was developed for the quantitative detection of chloramphenicol (CAP), thiamphenicol (TAP) and florfenicol (FF) in milk. The limit of detection (LOD) for CAP, TAP and FF in milk was 0.08, 0.8 and 1.9 μg L−1, respectively. The recovery of intra and inter-assay ranged from 87.7% to 107.0% and 82.5% to 112.3%, with corresponding coefficient of variations less than 11.3% and 14.5%, respectively. The sensitivity of the FMs lateral flow assay (FMs-LFA) was comparable to those of the colloidal gold lateral flow assay. A quantitative comparison of the FMs-LFA and LC–MS/MS analysis of milk samples indicated good agreement between the two methods. The FMs-LFA can be used as a reliable, rapid and cost-effective method for food safety analysis.

【 授权许可】

Unknown