Viruses | 卷:13 |
Long-Term Humoral Immune Response against SARS-CoV-2 after Natural Infection and Subsequent Vaccination According to WHO International Binding Antibody Units (BAU/mL) | |
Michael Schindler1  Natalia Ruetalo1  Karin Klingel2  Marija Reichenbächer3  Angelika Haage3  Andrea Normann3  Lutz Pridzun3  Bertram Flehmig4  Michael B. Ranke4  Teresa Kirchner5  Thomas Kirchner5  | |
[1] Institute for Medical Virology and Epidemiology, University Hospital Tuebingen, Elfriede-Aulhorn-Str. 6, 72076 Tuebingen, Germany; | |
[2] Institute for Pathology and Neuropathology, University Hospital Tuebingen, Liebermeisterstr. 8, 72076 Tuebingen, Germany; | |
[3] Mediagnost Gesellschaft für Forschung und Herstellung von Diagnostika GmbH, Aspenhaustr. 25, 72770 Reutlingen, Germany; | |
[4] Paediatric Endocrinology, University Children’s Hospital, Hoppe-Seyler-Str. 1, 72076 Tuebingen, Germany; | |
[5] Pediatric Practice, Kapuzinerberg 17, 71263 Weil der Stadt, Germany; | |
关键词: COVID-19; SARS-CoV-2; anti-SARS-CoV-2 antibodies; SARS-CoV-2 S1-RBD-protein; SARS-CoV-2 N-protein; SARS-CoV-2 WHO standard; | |
DOI : 10.3390/v13122336 | |
来源: DOAJ |
【 摘 要 】
The new WHO reference standard allows for the definition of serum antibodies against various SARS-CoV-2 antigens in terms of binding antibody units (BAU/mL) and thus to compare the results of different ELISA systems. In this study, the concentration of antibodies (ABs) against both the S- and the N-protein of SARS-CoV-2 as well as serum neutralization activity were evaluated in three patients after a mild course of COVID-19. Serum samples were collected frequently during a period of over one year. Furthermore, in two individuals, the effects of an additional vaccination with a mRNA vaccine containing the S1-RBD sequence on these antibodies were examined. After natural infection, the antibodies (IgA, IgG) against the S1-protein remained elevated above the established cut-off to positivity (S-IgA 60 BAU/mL and S-IgG 50 BAU/mL, respectively) for over a year in all patients, while this was not the case for ABs against the N-protein (cut-off N-IgG 40 BAU/mL, N-IgA 256 BAU/mL). Sera from all patients retained the ability to neutralize SARS-CoV-2 for more than a year. Vaccination resulted in a rapid boost of antibodies to S1-protein but, as expected, not to the N-protein. Most likely, the wide use of the WHO reference preparation will be very useful in determining the individual immune status of patients after an infection with SARS-CoV-2 or after vaccination.
【 授权许可】
Unknown