Long-Term Humoral Immune Response against SARS-CoV-2 after Natural Infection and Subsequent Vaccination According to WHO International Binding Antibody Units (BAU/mL)

Viruses

卷:13

Michael Schindler¹  Natalia Ruetalo¹  Karin Klingel²  Marija Reichenbächer³  Angelika Haage³  Andrea Normann³  Lutz Pridzun³  Bertram Flehmig⁴  Michael B. Ranke⁴  Teresa Kirchner⁵  Thomas Kirchner⁵ 

[1] Institute for Medical Virology and Epidemiology, University Hospital Tuebingen, Elfriede-Aulhorn-Str. 6, 72076 Tuebingen, Germany;

[2] Institute for Pathology and Neuropathology, University Hospital Tuebingen, Liebermeisterstr. 8, 72076 Tuebingen, Germany;

[3] Mediagnost Gesellschaft für Forschung und Herstellung von Diagnostika GmbH, Aspenhaustr. 25, 72770 Reutlingen, Germany;

[4] Paediatric Endocrinology, University Children’s Hospital, Hoppe-Seyler-Str. 1, 72076 Tuebingen, Germany;

[5] Pediatric Practice, Kapuzinerberg 17, 71263 Weil der Stadt, Germany;

关键词: COVID-19;    SARS-CoV-2;    anti-SARS-CoV-2 antibodies;    SARS-CoV-2 S1-RBD-protein;    SARS-CoV-2 N-protein;    SARS-CoV-2 WHO standard;   

DOI  :  10.3390/v13122336

来源: DOAJ

【 摘 要 】

The new WHO reference standard allows for the definition of serum antibodies against various SARS-CoV-2 antigens in terms of binding antibody units (BAU/mL) and thus to compare the results of different ELISA systems. In this study, the concentration of antibodies (ABs) against both the S- and the N-protein of SARS-CoV-2 as well as serum neutralization activity were evaluated in three patients after a mild course of COVID-19. Serum samples were collected frequently during a period of over one year. Furthermore, in two individuals, the effects of an additional vaccination with a mRNA vaccine containing the S1-RBD sequence on these antibodies were examined. After natural infection, the antibodies (IgA, IgG) against the S1-protein remained elevated above the established cut-off to positivity (S-IgA 60 BAU/mL and S-IgG 50 BAU/mL, respectively) for over a year in all patients, while this was not the case for ABs against the N-protein (cut-off N-IgG 40 BAU/mL, N-IgA 256 BAU/mL). Sera from all patients retained the ability to neutralize SARS-CoV-2 for more than a year. Vaccination resulted in a rapid boost of antibodies to S1-protein but, as expected, not to the N-protein. Most likely, the wide use of the WHO reference preparation will be very useful in determining the individual immune status of patients after an infection with SARS-CoV-2 or after vaccination.

【 授权许可】

Unknown