Somatic embryogenesis and plant regeneration of Capsicum baccatum L.

Journal of Genetic Engineering and Biotechnology

卷:14

Karampuri Subhash¹  Sadhu Suman Kalyan²  Pittampalli Bhanuprakash²  Peddaboina Venkataiah² 

[1] Department of Biotechnology, Kakatiya University, Warangal 506 009, India;

[2] Department of Microbiology, Kakatiya University, Warangal 506 009, India;

关键词: Cotyledon;    Leaf;    Pepper;    Somatic embryos;   

DOI  :  10.1016/j.jgeb.2016.02.001

来源: DOAJ

【 摘 要 】

A plant regeneration protocol via somatic embryogenesis was achieved in cotyledon and leaf explants of Capsicum baccatum, when cultured on MS medium supplemented with various concentrations of 2,4-dichlorophenoxy acetic acid (2,4-D, 0.5–5.0 mg l−1) in combination with Kinetin (Kn, 0.5 mg l−1) and 3% sucrose. Various stages were observed during the development of somatic embryos, including globular, heart, and torpedo-stages. Torpedo stage embryos were separated from the explants and subcultured on medium supplemented with various concentrations of different plant growth regulators for maturation. Maximum percentage (55%) of somatic embryo germination and plantlet formation was found at 1.0 mg l−1 BA. Finally, about 68% of plantlets were successfully established under field conditions. The regenerated plants were morphologically normal, fertile and able to set viable seeds.

【 授权许可】

Unknown