Agrobacterium tumefaciens-mediated transformation of modern rose (Rosa hybrida) using leaf-derived embryogenic callus

Horticultural Plant Journal

卷:7

Junping Gao¹  Hougao Zhou²  Ying Bao³  Guogui Ning⁴  Hui Jiang⁵  Xiaofeng Zhou⁵  Nan Ma⁵  Liangjun Zhao⁵  Yuan Yuan⁵  Guoqin Liu⁵ 

[1] Landscape Architecture, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;

[2] College Horticulture &

[3] Faculty of Life Science, Tangshan Normal University, Tangshan, Hebei 063000, China;

[4] Key Laboratory of Horticultural Plant Biology, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China;

[5] State Key Laboratory of Agrobiotechnology, Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture, College of Horticulture, China Agricultural University, Beijing 100193, China;

关键词: Rose;    Rosa hybrida;    Leaf;    Somatic embryos;    Agrobacterium tumefaciens;    Genetic transformation;   

DOI  :  

来源: DOAJ

【 摘 要 】

Rose (Rosa hybrida) is widely used for cut flowers and as garden plants. Stable and efficient transformation system is required for functional genomics of rose. Here, we established an efficient transformation method for rose using Agrobacterium tumefaciens-mediated transformation of embryogenic callus. Expanding rose leaves were used as explants to induce somatic embryos, which were subjected to transformation with A. tumefaciens strain GV3101 using Green Fluorescence Protein (GFP) as a marker gene. It took about 8 months to generate transgenic shoots from embryogenic callus. PCR, RT-PCR, Southern and Western blotting, as well as stereoscopic fluorescence microscopy analysis demonstrated that GFP transgenes integrated stably into the rose genome. According to our data, a transformation efficiency of up to 6% can be achieved by following this optimized protocol.

【 授权许可】

Unknown