Early Immunological Effects of Ischemia-Reperfusion Injury: No Modulation by Ischemic Preconditioning in a Randomised Crossover Trial in Healthy Humans

International Journal of Molecular Sciences

卷:20

BoMartin Bibby¹  Marco Eijken²  MikkelSteen Petersen²  ThomasH. Lange²  BjarneK. Møller²  Carla Baan³  Bente Jespersen⁴ 

[1] Department of Biostatistics, University of Aarhus, Bartholins Allé 2, 8000 Aarhus C, Denmark;

[2] Department of Clinical Immunology, Aarhus University Hospital, Palle Juul-Jensens Blvd. 99, 8200 Aarhus N, Denmark;

[3] Department of Internal Medicine, Sector Nephrology and Transplantation, Erasmus Medical Center, Doctor Molewaterplein 40, 3015 GD Rotterdam, The Netherlands;

[4] Department of Renal Diseases, Aarhus University Hospital, Palle Juul-Jensens Blvd. 99, 8200 Aarhus N, Denmark;

关键词: transplantation immunology;    ischemia reperfusion injury;    ischemic conditioning;    immune regulation;    early immune response;    flow cytometry;   

DOI  :  10.3390/ijms20122877

来源: DOAJ

【 摘 要 】

Ischemic preconditioning (IPC) has been protective against ischemia-reperfusion injury (IRI), but the underlying mechanism is poorly understood. We examined whether IPC modulates the early inflammatory response after IRI. Nineteen healthy males participated in a randomised crossover trial with and without IPC before IRI. IPC and IRI were performed by cuff inflation on the forearm. IPC consisted of four cycles of five minutes followed by five minutes of reperfusion. IRI consisted of twenty minutes followed by 15 min of reperfusion. Blood was collected at baseline, 0 min, 85 min and 24 h after IRI. Circulating monocytes, T-cells subsets and dendritic cells together with intracellular activation markers were quantified by flow cytometry. Luminex measured a panel of inflammation-related cytokines in plasma. IRI resulted in dynamic regulations of the measured immune cells and their intracellular activation markers, however IPC did not significantly alter these patterns. Neither IRI nor the IPC protocol significantly affected the levels of inflammatory-related cytokines. In healthy volunteers, it was not possible to detect an effect of the investigated IPC-protocol on early IRI-induced inflammatory responses. This study indicates that protective effects of IPC on IRI is not explained by direct modulation of early inflammatory events.

【 授权许可】

Unknown