CdS Nanoparticle-based Biosensor Development for Aflatoxin Determination

International Journal of Technology

卷:10

Tribidasari A Ivandini¹  Moh Hayat¹  Endang Saepudin¹  Yasuaki Einaga² 

[1] Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Indonesia, Kampus UI Depok 16424, Indonesia;

[2] Department of Chemistry, Keio University, Japan;

关键词: Aflatoxin;    Anodic stripping voltammetry;    Boron-doped diamond;    CdS nanoparticles;   

DOI  :  10.14716/ijtech.v10i4.2407

来源: DOAJ

【 摘 要 】

An anodic strippingvoltammetry detection method with lateral flow immunochromatographic assay fordetermining aflatoxin M1 was developed. Cadmium sulfide nanoparticles (CdSNPs) were employed as a label for aflatoxin antibody. Conjugation between the CdSNPsand aflatoxin antibody was performed as the first step. The CdSNP-antibodyconjugate was then immobilized on a test strip on a conjugate pad. When the conjugateinteracted with the sample containing aflatoxin, it was captured by the aflatoxinin the test zone. The CdSNPs retained in the test zone were then measured by anodic strippingvoltammetry using a boron-doped diamond electrode. A linear concentration rangeof 0–70 ppb aflatoxin, with a sensitivity of 20 µA/mM and a detection limit of 30 ppb,was achieved by this method.

【 授权许可】

Unknown