Association of salivary Cathepsin B in different histological grades among patients presenting with oral squamous cell carcinoma

BMC Oral Health

卷:22

Humera Waheed¹  Sabhita Shabir Shaikh²  Alveena Shabbir³  Shaheen Ahmed⁴  Waqas Ahmed Farooqui⁵ 

[1] Department of Dow College of Biotechnology, Dow University of Health Sciences;

[2] Department of NILGID, Dow University of Health Sciences;

[3] Department of Oral Medicine, Dow University of Health Sciences;

[4] Department of Oral and Maxillofacial Surgery, Dow University of Health Sciences;

[5] Department of School of Public Health, Dow University of Health Sciences;

关键词: Oral squamous cell carcinoma;    Saliva;    ELISA;    Cathepsin B;    Biomarker;    Histological grades;   

DOI  :  10.1186/s12903-022-02052-1

来源: DOAJ

【 摘 要 】

Abstract Background Oral cancer is considered a major public health problem due to its high mortality and morbidity rates. Survival rate of OSCC can be significantly improved by using non-invasive tool such as salivary biomarkers for detection of OSCC which is considered a promising approach. Cathepsin B is a lysosomal cysteine protease, present in abundant quantities in lysosome of cells, tissues and different biological fluids. Increased expression of Cathepsin B was observed in many malignancies including oral cancer. The present study was designed to determine the salivary levels of Cathepsin B in different histological grades of OSCC. Method In this study, total no. of 80 research participants were enrolled which were divided into four groups. Each group comprised 20 participants, group 1 comprised 20 patients of OSCC (well differentiated), group 2 comprised 20 patients of OSCC (moderately differentiated), group 3 comprised 20 patients of OSCC (poorly differentiated) and group 4 comprised 20 healthy controls. Saliva sample was collected from all the four study groups and salivary Cathepsin B levels were analyzed by ELISA sandwich technique in duplicate. Results Salivary levels of Cathepsin B were significantly increased with p value (< 0.001) in patients of OSCC as compared to control group according to both histological grades and tumor size. Highest mean Cathepsin B levels in well differentiated OSCC followed by poorly differentiated OSCC and moderately differentiated OSCC were observed. Conclusion Results of the present study suggests that Cathepsin B has a great value as a salivary biomarker for diagnosis and monitoring of OSCC in different histological grades. This will further lead to increase survival rate and improve the prognosis of OSCC.

【 授权许可】

Unknown