Changes to interneuron-driven striatal microcircuits in a rat model of Parkinson's disease

Neurobiology of Disease

卷:34

Lydia Kerkerian-Le Goff¹  Pascal Salin¹  Philippe Kachidian¹  Patrice Coulon²  Alberto J. Rico³  Pedro Barroso-Chinea³  Iciar P. López³  José L. Lanciego³  Virginia Gómez-Bautista³ 

[1] Developmental Biology Institute of Marseille-Luminy, UMR 6216 CNRS-Université de la Méditerranée, Marseille, France;

[2] Laboratoire de la Plasticité et Physio-Pathologie de la Motricité, UMR 6196 CNRS-Université de la Méditerranée, Marseille, France;

[3] Neurosciences Division, Center for Applied Medical Research (CIMA and CIBERNED), University of Navarra Medical College, Pamplona, Spain;

关键词: Rabies virus;    ChAT;    Dopamine;    Caudate–putamen;    Basal ganglia;   

DOI  :  

来源: DOAJ

【 摘 要 】

Striatal interneurons play key roles in basal ganglia function and related disorders by modulating the activity of striatal projection neurons. Here we have injected rabies virus (RV) into either the rat substantia nigra pars reticulata or the globus pallidus and took advantage of the trans-synaptic spread of RV to unequivocally identify the interneurons connected to striatonigral- or striatopallidal-projecting neurons, respectively. Large numbers of RV-infected parvalbumin (PV+/RV+) and cholinergic (ChAT+/RV+) interneurons were detected in control conditions, and they showed marked changes following intranigral 6-hydroxydopamine injection. The number of ChAT+/RV+ interneurons innervating striatopallidal neurons increased concomitant with a reduction in the number of PV+/RV+ interneurons, while the two interneuron populations connected to striatonigral neurons were clearly reduced. These data provide the first evidence of synaptic reorganization between striatal interneurons and projection neurons, notably a switch of cholinergic innervation onto striatopallidal neurons, which could contribute to imbalanced striatal outflow in parkinsonian state.

【 授权许可】

Unknown