Specific lysis of GABAergic synaptosomes by an antiserum to glutamate decarboxylase

FEBS Letters

Bradford, Henry F.³  Anderton, Brian¹  Docherty, Maureen³  Wu, Jang-Yen² 

[1] Department of Immunology, St. Georges Hospital Medical School, Cranmer Terrace, London SW17 0RE, England;Department of Cell Biology, Baylor College of Medicine, Texas Medical Center, Houston TX 77030, USA;Department of Biochemistry, Imperial College, London SW7 2AZ, England

关键词: Synaptosome;    GABAergic;    Lysis;    Antiserum;    GAD;    LDH;    BSA;    bovine serum albumin;    GAD;    glutamate decarboxylase (EC 4.1.1.15);    ChAT;    choline acetyltransferase (EC 2.3.1.6);    LDH;    lactate dehydrogenase (EC 1.1.1.27);    GABA;    γ-aminobutyric acid;    NA;    noradrenaline;    DABA;    diaminobutyric acid;    Butyl-PBD;    2-(41-tert-Butylphenyl)-5-(411-biphenyl)-1;    3;    4-oxadiazole;   

DOI  :  10.1016/0014-5793(83)80481-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An antiserum to pure glutamate decarboxylase (CGAD) when incubated with rat cortical synaptosomes in the presence of complement caused release of 33–53% of lactate dehydrogenase (LDH) and 22–41% of total GAD. In addition most of the γ-aminobutyrate (GABA) present was released. Anti-GAD antiserum alone, or complement alone, were without action. The antiserum plus complement had no effect on noradrenaline or choline uptake, and did not release cholineacetylase (ChAT). Anti-ChAT serum plus complement released 30–37% of ChAT and 10–13% of LDH. It prevented choline uptake. This serum did not produce GAD release or prevent GABA, choline or noradrenaline uptake. When cortical synaptosomes were exposed to both antisera plus complement, their actions were strictly additive. The data indicate specific lysis of GABAergic and cholinergic synaptosomal sub-populations.

【 授权许可】

Unknown