Specific immunolysis of serotonergic nerve terminals using an antiserum against tryptophan hydroxylase

FEBS Letters

Cash, Christopher D.²  Bradford, H.F.¹  Docherty, Maureen¹  Maitre, Michel² 

[1] Department of Biochemistry, Imperial College of Science and Technology, South Kensington, London SW7 2AZ, England;Centre de Neurochimie du CNRS, 5, rue Blaise Pascal, 67084 Strasbourg Cedex, France

关键词: Synaptosome;    Serotonergic nerve terminal;    Lysis;    Antiserum;    Tryptophan hydroxylase;   

DOI  :  10.1016/0014-5793(85)80360-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An antiserum to tryptophan hydroxylase purified from whole rat brain when incubated with rat striatal synaptosomes in the presence of complement caused release of 18% of LDH, 20% loss of potassium and 60% loss of tryptophan hydroxylase. Uptake of 5-HT was reduced by 60%. Anti-tryptophan hydroxylase alone, or complement alone were without action. The antiserum plus complement had no effect on DA uptake and did not release TH or GAD. These results suggest selective lysis of serotonergic nerve terminals had occurred. The antiserum plus complement reduced choline uptake by 45%. However, this did not seem due to lysis of cholinergic terminals, as ChAT was not released.

【 授权许可】

Unknown