Sugary Endosperm is Modulated by Starch Branching Enzyme IIa in Rice (Oryza sativa L.)

Rice

卷:10

Mi-Ra Yoon¹  Gileung Lee²  Mi-Ok Woo²  Yunjoo Lee²  Backki Kim²  Hee-Jong Koh²  Su Jang²  Joong Hyoun Chin³  Rihua Piao⁴  Min-Seon Choi⁵ 

[1] Department of Central Area Crop Science, National Institute of Crop Science (NICS), RDA;

[2] Department of Plant Science and Research Institute for Agriculture and Life Sciences, and Plant Genomics and Breeding Institute, Seoul National University;

[3] Graduate School of Integrated Bioindustry, Sejong University;

[4] Rice Research Institute, Jilin Academy of Agricultural Sciences;

[5] Vegetable Crop Division, National Institute of Horticultural and Herbal Science, Rural Development Administration;

关键词: Map-based cloning;    OsBEIIa;    OsISA1;    Rice;    Sugary-h;    Sugary endosperm;   

DOI  :  10.1186/s12284-017-0172-3

来源: DOAJ

【 摘 要 】

Abstract Background Starch biosynthesis is one of the most important pathways that determine both grain quality and yield in rice (Oryza sativa L.). Sugary endosperm, sugary-1 (sug-1), is a mutant trait for starch biosynthesis. Rice plants carrying sug-1 produce grains that accumulate water-soluble carbohydrates instead of starch, even after maturity. Although this trait enhances the diversity of grain quality, sugary endosperm rice has hardly been commercialized due to the severely wrinkled grains and subsequent problems in milling. This study was conducted to identify the genes responsible for the sug-h phenotype through a map-based cloning technology. Results We induced a mild sugary mutant, sugary-h (sug-h) through the chemical mutagenesis on the Korean japonica cultivar Hwacheong. Grains of the sug-h mutant were translucent and amber-colored, and the endosperm appeared less wrinkled than sug-1, whereas the soluble sugar content was fairly high. These characteristics confer greater marketability to the sug-h mutant. Genetic analyses indicated that the sug-h mutant phenotype was controlled by a complementary interaction of two recessive genes, Isoamylase1 (OsISA1), which was reported previously, and Starch branching enzyme IIa (OsBEIIa), which was newly identified in this study. Complementation tests indicated that OsBEIIa regulated the properties of sugary endosperm. Conclusions Complementary interactions between the starch biosynthesis genes OsISA1 and OsBEIIa determine the mild sugary endosperm mutant, sugary-h, in rice. Our finding may facilitate the breeding of sugaryendosperm rice for commercial benefit.

【 授权许可】

Unknown