Monitoring the Levels of Cellular NF-κB Activation States

Cancers

卷:13

Jan Dreute¹  M. Lienhard Schmitz¹  Argyris Papantonis²  Uwe Linne³  Lisa Leib⁴  Michael Kracht⁴  Johanna Meier-Soelch⁴  Christin Mayr-Buro⁴  Jana Juli⁴ 

[1] Institute of Biochemistry, Justus Liebig University, 35392 Giessen, Germany;

[2] Institute of Pathology, University Medical Center Göttingen, 37075 Göttingen, Germany;

[3] Mass Spectrometry Facility of the Department of Chemistry, Philipps University, 35032 Marburg, Germany;

[4] Rudolf Buchheim Institute of Pharmacology, Justus Liebig University, 35392 Giessen, Germany;

关键词: NF-κB;    signaling;    kinase;    transcription factor;    methods;    single cell assays;   

DOI  :  10.3390/cancers13215351

来源: DOAJ

【 摘 要 】

The NF-κB signaling system plays an important regulatory role in the control of many biological processes. The activities of NF-κB signaling networks and the expression of their target genes are frequently elevated in pathophysiological situations including inflammation, infection, and cancer. In these conditions, the outcome of NF-κB activity can vary according to (i) differential activation states, (ii) the pattern of genomic recruitment of the NF-κB subunits, and (iii) cellular heterogeneity. Additionally, the cytosolic NF-κB activation steps leading to the liberation of DNA-binding dimers need to be distinguished from the less understood nuclear pathways that are ultimately responsible for NF-κB target gene specificity. This raises the need to more precisely determine the NF-κB activation status not only for the purpose of basic research, but also in (future) clinical applications. Here we review a compendium of different methods that have been developed to assess the NF-κB activation status in vitro and in vivo. We also discuss recent advances that allow the assessment of several NF-κB features simultaneously at the single cell level.

【 授权许可】

Unknown