Prenatal choline supplementation attenuates neuropathological response to status epilepticus in the adult rat hippocampus

Neurobiology of Disease

卷:30

Tiffany J. Mellott¹  Jan K. Blusztajn¹  Melissa J. Glenn²  Sarah J.E. Wong-Goodrich²  Christina L. Williams² 

[1] Department of Pathology and Laboratory Medicine, Boston University School of Medicine, Boston, MA, 02118, USA;

[2] Department of Psychology and Neuroscience, Duke University, Durham, NC 27708, USA;

关键词: Choline;    Kainic acid;    Hippocampus;    Seizures;    Bromodeoxyuridine;    Growth factor;   

DOI  :  

来源: DOAJ

【 摘 要 】

Prenatal choline supplementation (SUP) protects adult rats against spatial memory deficits observed after excitotoxin-induced status epilepticus (SE). To examine the mechanism underlying this neuroprotection, we determined the effects of SUP on a variety of hippocampal markers known to change in response to SE and thought to underlie ensuing cognitive deficits. Adult offspring from rat dams that received either a control or SUP diet on embryonic days 12–17 were administered saline or kainic acid (i.p.) to induce SE and were euthanized 16 days later. SUP markedly attenuated seizure-induced hippocampal neurodegeneration, dentate cell proliferation, and hippocampal GFAP mRNA expression levels, prevented the loss of hippocampal GAD65 protein and mRNA expression, and altered growth factor expression patterns. SUP also enhanced pre-seizure hippocampal levels of BDNF, NGF, and IGF-1, which may confer a neuroprotective hippocampal microenvironment that dampens the neuropathological response to and/or helps facilitate recovery from SE to protect cognitive function.

【 授权许可】

Unknown