Exploration of a panel of urine biomarkers of kidney disease in two paediatric cohorts with Type 1 diabetes mellitus of differing duration

Diabetology & Metabolic Syndrome

卷:14

Keith Burling¹  Carolina Canepa²  Robert J. Unwin³  Anthony G. W. Norden³  Letizia Zeni⁴  Giovanni Cancarini⁵  Barbara Felappi⁶  Alessandro Plebani⁶  Elena Prandi⁶  Pietro Manuel Ferraro⁷  Donald Fraser⁸  Katherine Simpson⁸ 

[1] Core Biochemical Assay Laboratory, Cambridge University Hospitals NHS Foundation Trust;

[2] Department of Nephrology, Royal Berkshire Hospital;

[3] Department of Renal Medicine, Royal Free Hospital Trust, University College London;

[4] Nephrology Unit, Azienda Socio-Sanitaria Territoriale degli Spedali Civili di Brescia, Piazzale Spedali Civili;

[5] Nephrology, Department of Medical and Surgical Specialties, Radiological Science and Public Health, University of Brescia;

[6] Pediatrics Clinic, Department of Clinical and Experimental Sciences, University of Brescia and ASST Spedali Civili of Brescia;

[7] U.O.S. Terapia Conservativa della Malattia Renale Cronica, U.O.C. Nefrologia, Fondazione Policlinico Universitario A. Gemelli IRCCS;

[8] Wales Kidney Research Unit, Division of Infection and Immunity, School of Medicine, College of Biomedical and Life Sciences, Cardiff University;

关键词: Diabetic kidney disease;    Urinary biomarkers;    Type 1 diabetes mellitus;    Albuminuria;    microRNAs;    Urine free retinol-binding protein 4;   

DOI  :  10.1186/s13098-022-00839-4

来源: DOAJ

【 摘 要 】

Abstract Background The pathogenesis of diabetic kidney disease (DKD) is complex and involves both glomerular and tubular dysfunction. A global assessment of kidney function is necessary to stage DKD, a progressive kidney disease that is likely to begin in childhood. The present study evaluated whether kidney injury biomarkers identified as early DKD biomarkers in adults have any prognostic value in the very early stages of childhood diabetes. Methods We measured urine free Retinol-binding protein 4 (UfRBP4), albumin (UAlb), Kidney injury molecule-1 (KIM-1) and the microRNAs miR-155, miR-126 and miR-29b in two cohorts of paediatric T1DM patients without evidence of DKD, but with diabetes of short-duration, ≤ 2.5 years (SD, n = 25) or of long-duration, ≥ 10 years (LD, n = 29); non-diabetic siblings (H, n = 26) were recruited as controls. A p value < 0.05 was considered significant for all results. Results UfRBP4 and UAlb were not significantly different across the three groups. No differences were found in KIM-1 excretion between any of the three groups. UfRBP4 was correlated with UAlb in all three groups (r 0.49; p < 0.001), whereas KIM-1 showed no correlation with albumin excretion. Among microRNAs, miR-29b was higher in all diabetic children compared with the H control group (p = 0.03), whereas miR-155 and miR-126 were not significantly different. No differences were found between the SD and LD groups for all three microRNAs. No associations were identified between these biomarkers with sex, age, BMI, eGFR, T1DM duration or glycaemic control. Conclusions UfRBP4, KIM-1, miR-155, and miR-126 were unaffected by the presence and duration of diabetes, whereas miR-29b showed a modest elevation in diabetics, regardless of duration. These data support the specificity of a panel of urine biomarkers as DKD biomarkers, rather than any relationship to diabetes per se or its duration, and not as early DKD biomarkers in a paediatric setting.

【 授权许可】

Unknown