Vitrification and Subsequent In Vitro Maturation of Mouse Preantral Follicles in Presence of Growth Factors

Cell Journal

卷:16

关键词: Vitrification;    Mouse Preantral Follicle;    In Vitro Maturation;    Epidermal Growth Factor;    Fibroblast Growth Factor;   

DOI  :  

来源: DOAJ

【 摘 要 】

Objective: Cryopreservation of ovarian tissue or follicles has been proposed as analternative method for fertility preservation. Although successful vitrification of follicleshas been reported in several mammalian species, the survival rate is generallylow. The aim of this study was to investigate the effects of fibroblast growth factor(FGF) and epidermal growth factor (EGF) on in vitro preantral follicle developmentafter vitrification.Materials and Methods:In this experimental study, preantral follicles with diameter of150-180 μm were mechanically isolated from ovaries of 18-21 days old NMRI mice. Follicleswere vitrified and warmed, then cultured in α-minimal essential medium (α-MEM)without growth factor supplementation as control group (group I), while supplementedwith 20 ng/ml FGF (group II), 20 ng/ml EGF (group III), and 20 ng/ml FGF +20 ng/ml EGF(group IV). After 12 days, human chorionic gonadotrophin (hCG)/EGF was added to culturemedium, and after 18-20 hours, the presence of cumulus oocyte complexes (COCs)and oocyte maturation were assessed. The chi-square (χ2) test was used to analyze survivaland ovulation rates of the follicles.Results: Our results showed that the rate of metaphase II (MII) oocytes in FGF groupincreased in comparison with control and other treatment groups (p<0.027), but therewas no difference between control with EGF and EGF+FGF groups in oocyte maturationrate (p>0.05). There was a significant decrease in survival rate of follicles inEGF+FGE group in comparison with other groups (p<0.008). After in vitro ovulationinduction, the follicles in EGF group showed a higher ovulation rate (p<0.008) thanthose cultured in other groups.Conclusion: FGF has beneficial effect on oocyte maturation, and EGF increasesCOCs number in vitro. Combination of EGF and FGE decreases the number of survivedfollicles.

【 授权许可】

Unknown