Honokiol ameliorates oxidative stress-induced DNA damage and apoptosis of c2c12 myoblasts by ROS generation and mitochondrial pathway

Animal Cells and Systems

卷:24

Hyesook Lee¹  Su Hyun Hong¹  Yung Hyun Choi¹  Sung-Kwon Moon²  Gi-Young Kim³  Cheol Park⁴  Sung Hyun Choi⁵  Wun-Jae Kim⁶  Jin-Woo Jeong⁷  Min Ho Han⁸ 

[1] Department of Biochemistry, Dong-eui University College of Korean Medicine;

[2] Department of Food and Nutrition, Chung-Ang University;

[3] Department of Marine Life Sciences, Jeju National University;

[4] Department of Molecular Biology, College of Natural Sciences, Dong-eui University;

[5] Department of System Management, Korea Lift College;

[6] Department of Urology, College of Medicine, Chungbuk National University;

[7] Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources;

[8] National Marine Biodiversity Institute of Korea;

关键词: honokiol;    oxidative stress;    dna damage;    apoptosis;    ros;   

DOI  :  10.1080/19768354.2019.1706634

来源: DOAJ

【 摘 要 】

Honokiol is one of the main active components of Magnolia officinalis, and has been demonstrated to have multiple pharmacological activities against a variety of diseases. Recently, this phenolic compound is known to have antioxidant activity, but its mechanism of action remains unclear. The purpose of the current study was to evaluate the preventive effects of honokiol against oxidative stress-induced DNA damage and apoptosis in C2C12 myoblasts. The present study found that honokiol inhibited hydrogen peroxide (H2O2)-induced DNA damage and mitochondrial dysfunction, while reducing reactive oxygen species (ROS) formation. The inhibitory effect of honokiol on H2O2-induced apoptosis was associated with the up-regulation of Bcl-2 and down-regulation of Bax, thus reducing the Bax/Bcl-2 ratio that in turn protected the activation of caspase-9 and -3, and inhibition of poly (ADP-ribose) polymerase cleavage, which was associated with the blocking of cytochrome c release to the cytoplasm. Collectively, these results demonstrate that honokiol defends C2C12 myoblasts against H2O2-induced DNA damage and apoptosis, at least in part, by preventing mitochondrial-dependent pathway through scavenging excessive ROS.

【 授权许可】

Unknown