Functional Divergence of G and Its Homologous Genes for Green Pigmentation in Soybean Seeds

Frontiers in Plant Science

卷:12

Hiroki Sasada¹  Haruna Nakano¹  Ayaka Hase¹  Taiken Nakashima²  Takuto Kozu²  Tetsuya Yamada²  Jun Abe²  Yusuke Tokumitsu²  Makoto Kusaba³  Takeshi Ito³  Hiroshi Yamatani⁴  Akito Kaga⁴  Masao Ishimoto⁴  Yoshitake Takada⁵ 

[1] Faculty of Agriculture, Hokkaido University, Sapporo, Japan;

[2] Graduate School of Agriculture, Hokkaido University, Sapporo, Japan;

[3] Graduate School of Science, Hiroshima University, Higashihiroshima, Japan;

[4] Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba, Japan;

[5] Western Region Agricultural Research Center, National Agriculture and Food Research Organization, Fukuyama, Japan;

关键词: Glycine max;    chlorophyll;    seed coat;    wild soybean (G. soja Sieb. and Zucc.);    landraces;    domestication;   

DOI  :  10.3389/fpls.2021.796981

来源: DOAJ

【 摘 要 】

The degradation of chlorophyll in mature soybean seeds is closely related to the development of their yellow color. In this study, we examined G, its homologue G-like (GL), and their mutant alleles and investigated the relationship between these genes and chlorophyll accumulation in the seed coats of mature seeds. Transient expression of G and GL proteins fused with green fluorescent protein revealed that both were localized in plastids. Overexpression of G resulted in the accumulation of chlorophyll in the seed coats and cotyledons of mature seeds, indicating that high expression levels of G result in chlorophyll accumulation that exceeds its metabolism in the seeds of yellow soybean. Analysis of near isogenic lines at the G locus demonstrated a significant difference in the chlorophyll content of the seed coats and cotyledons of mature seeds when G and mutant g alleles were expressed in the d1d2 stay-green genetic background, indicating that the G protein might repress the SGR-independent degradation of chlorophyll. We examined the distribution of mutant alleles at the G and GL loci among cultivated and wild soybean germplasm. The g allele was widely distributed in cultivated soybean germplasm, except for green seed coat soybean lines, all of which contained the G allele. The gl alleles were much fewer in number than the g alleles and were mainly distributed in the genetic resources of cultivated soybean from Japan. None of the landraces and breeding lines investigated in this study were observed to contain both the g and gl alleles. Therefore, in conclusion, the mutation of the G locus alone is essential for establishing yellow soybeans, which are major current soybean breeding lines.

【 授权许可】

Unknown