Comparative Study of the Antioxidant and Enzyme Inhibitory Activities of Two Types of Moroccan Euphorbia Entire Honey and Their Phenolic Extracts

Foods

卷:10

Brígida Rodrigues¹  Jorge Carlier²  Soukaina El-Guendouz³  Sara Raposo³  Maria Graça Miguel³  Oumaima Boutoub³  Maria Clara Costa³  Lahsen El Ghadraoui⁴  Smail Aazza⁵  Ana Manhita⁶  Cristina Barrocas Dias⁶  Letícia M. Estevinho⁷  Vanessa B. Paula⁷ 

[1] CIMA—Centre for Marine and Environmental Research, FCT, Campus de Gambelas, Universidade do Algarve, 8005-139 Faro, Portugal;

[2] Centre of Marine Sciences (CCMAR), Gambelas Campus, University of the Algarve, 8005-139 Faro, Portugal;

[3] Faculdade de Ciências e Tecnologia, Campus de Gambelas, Universidade do Algarve, 8005-139 Faro, Portugal;

[4] Laboratory of Functional Ecology and Environment, Faculty of Science and Technology, BP 2202, University Sidi Mohamed Ben Abdallah, Fez 30000, Morocco;

[5] Laboratory of Phytochemistry, National Agency of Medicinal and Aromatic Plants (ANPMA), BP 159, Principal, Taounate 34000, Morocco;

[6] Laboratório HERCULES, Instituto de Investigação e Formação Avançada, Universidade de Évora, Largo Marquês de Marialva 8, 7000-809 Évora, Portugal;

[7] Mountain Research Center (CIMO), Polytechnic Institute of Bragança, Campus Santa Apolónia, 5300-253 Bragança, Portugal;

关键词: honey;    phenolic compounds;    antioxidant activity;    Euphorbia resinifera;    Euphorbia officinarum;    phenolic profile;   

DOI  :  10.3390/foods10081909

来源: DOAJ

【 摘 要 】

Honey is a natural food product very famous for its health benefits for being an important source of antioxidant and phenolic compounds. Euphorbia honeys obtained from different regions of Morocco were evaluated for their ability to inhibit acetylcholinesterase, lipoxygenase, tyrosinase and xanthine oxidase activities. Their antioxidant properties were evaluated using the: 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging capacity, nitric oxide scavenging activity (NO) and scavenging ability of superoxide anion radical. Then, the phenolic extracts of the same entire honey samples were evaluated by liquid chromatography coupled to diode array detection and mass spectrometry (LC-DAD-MS) and tested for the biological activities previously evaluated on the entire honeys, in order to conduct a comparative study between both (honey and phenolic extracts). The chromatographic profiles for the studied Euphorbia honey extracts were different. Phenolic compounds gallic acid, 4-hydroxybenzoic acid and p-coumaric acid were detected in all samples, whereas kampferol was only present in two samples. Physicochemical parameters and total phenolic content were also determined. Entire honey that recorded the highest rate of phenols was sample M6 (E. resinifera) = 69.25 mg GAE/100 g. On the other hand, the phenolic extracts had better antioxidant and enzyme inhibitory activities than the entire honeys, regardless the monofloral honey type. In conclusion, the studied Euphorbia honeys may have a great potential as antioxidant, anti-inflammatory and anti-tyrosinase sources for pharmaceutical and cosmetic applications.

【 授权许可】

Unknown