Ultra Low-Dose Radiation: Stress Responses and Impacts Using Rice as a Grass Model

International Journal of Molecular Sciences

卷:10

关键词: Contaminated soil;    γ-ray;    Marker genes;    Oryza sativa;    Phytoalexins;   

DOI  :  10.3390/ijms10031215

来源: DOAJ

【 摘 要 】

We report molecular changes in leaves of rice plants (Oryza sativa L. - reference crop plant and grass model) exposed to ultra low-dose ionizing radiation, first using contaminated soil from the exclusion zone around Chernobyl reactor site. Results revealed induction of stress-related marker genes (Northern blot) and secondary metabolites (LC-MS/MS) in irradiated leaf segments over appropriate control. Second, employing the same in vitro model system, we replicated results of the first experiment using in-house fabricated sources of ultra low-dose gamma (g) rays and selected marker genes by RT-PCR. Results suggest the usefulness of the rice model in studying ultra low-dose radiation response/s.

【 授权许可】

Unknown