Ultra Low-Dose Radiation: Stress Responses and Impacts Using Rice as a Grass Model

International Journal of Molecular Sciences

Randeep Rakwal²  Ganesh Kumar Agrawal⁴  Junko Shibato²  Tetsuji Imanaka¹  Satoshi Fukutani¹  Shigeru Tamogami⁶  Satoru Endo⁵  Sarata Kumar Sahoo³  Yoshinori Masuo² 

[1] Research Reactor Institute, Kyoto University (KURRI), Osaka, Japan; E-Mails:;Health Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST), West, Tsukuba, Ibaraki 305–8569, Japan; E-Mails:;Research Center for Radiation Protection, National Institute of Radiological Sciences (NIRS), Chiba 263–8555, Japan; E-Mail:;Research Laboratory for Biotechnology and Biochemistry (RLABB), GPO Box 8207, Kathmandu, Nepal; E-Mails:;Graduate School of Engineering, Hiroshima University (HU), Hiroshima, Japan; E-Mail:;Laboratory of Growth Regulation Chemistry, Akita Prefectural University, Akita 010–0195, Japan; E-Mail:

关键词: Contaminated soil;    γ-ray;    Marker genes;    Oryza sativa;    Phytoalexins;   

DOI  :  10.3390/ijms10031215

来源: mdpi

【 摘 要 】

We report molecular changes in leaves of rice plants (Oryza sativa L. - reference crop plant and grass model) exposed to ultra low-dose ionizing radiation, first using contaminated soil from the exclusion zone around Chernobyl reactor site. Results revealed induction of stress-related marker genes (Northern blot) and secondary metabolites (LC-MS/MS) in irradiated leaf segments over appropriate control. Second, employing the same in vitro model system, we replicated results of the first experiment using in-house fabricated sources of ultra low-dose gamma (γ) rays and selected marker genes by RT-PCR. Results suggest the usefulness of the rice model in studying ultra low-dose radiation response/s.

【 授权许可】

CC BY   
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).