Embryonic echocardiography for assessment of congenital and functional cardiac defects

STAR Protocols

卷:2

Beatriz Escobar¹  Maria Villalba-Orero²  Ivan Menendez-Montes³  Silvia Martin-Puig³ 

[1] Corresponding author;

[2] Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX, USA;

[3] Vascular Pathophysiology. National Center for Cardiovascular Research. Madrid, Spain;

关键词: Developmental biology;    Health Sciences;    Model Organisms;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Cardiac function and morphology by mouse fetal echocardiography can be assessed by scanning the uterus extracted from the abdominal cavity (trans-uterine ultrasound) or the womb (trans-abdominal ultrasound). Advantages of trans-abdominal ultrasound include (1) non-invasive longitudinal analysis at different stages, reducing animal use; and (2) maintenance of natural environment, diminishing perturbations on functional parameters, which are more frequent in trans-uterine conditions. Here we describe both approaches, explaining how to identify congenital cardiac defects and defining the correlation between echocardiography findings and histological analysis.For complete details on the use and execution of this protocol, please refer to (Menendez-Montes et al., 2016) and (Menendez-Montes et al., 2021).

【 授权许可】

Unknown