Preanalytical Stability of CSF Total and Oligomeric Alpha-Synuclein

Frontiers in Aging Neuroscience

卷:13

Brit Mollenhauer¹  Eline A. J. Willemse²  Charlotte E. Teunissen²  Omar M. El-Agnaf³  Nour K. Majbour³  Ilham Y. Abdi³  Wilma D. J. van de Berg⁵ 

[1] Department of Neurology, University Medical Center Göttingen, Göttingen, Germany;

[2] Neurochemistry Laboratory, Department of Clinical Chemistry, Amsterdam Neuroscience, Amsterdam University Medical Center, Amsterdam, Netherlands;

[3] Neurological Disorders Research Centre, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Doha, Qatar;

[4] Paracelsus-Elena-Klinik, Klinikstraße, Kassel, Germany;

[5] Section Clinical Neuroanatomy and Biobanking, Department of Anatomy and Neurosciences, Amsterdam Neuroscience, Amsterdam University Medical Center, Vrije University Amsterdam, Amsterdam, Netherlands;

关键词: preanalytical factors;    alpha-synuclein;    oligomers;    cerebrospinal fluid;    biomarkers;   

DOI  :  10.3389/fnagi.2021.638718

来源: DOAJ

【 摘 要 】

Background: The role of cerebrospinal fluid (CSF) alpha-synuclein as a potential biomarker has been challenged mainly due to variable preanalytical measures between laboratories. To evaluate the impact of the preanalytical factors contributing to such variability, the different subforms of alpha-synuclein need to be studied individually.Method: We investigated the effect of exposing CSF samples to several preanalytical sources of variability: (1) different polypropylene (PP) storage tubes; (2) use of non-ionic detergents; (3) multiple tube transfers; (4) multiple freeze-thaw cycles; and (5) delayed storage. CSF oligomeric- and total-alpha-synuclein levels were estimated using our in-house sandwich-based enzyme-linked immunosorbent assays.Results: Siliconized tubes provided the optimal preservation of CSF alpha-synuclein proteins among other tested polypropylene tubes. The use of tween-20 detergent significantly improved the recovery of oligomeric-alpha-synuclein, while multiple freeze-thaw cycles significantly lowered oligomeric-alpha-synuclein in CSF. Interestingly, oligomeric-alpha-synuclein levels remained relatively stable over multiple tube transfers and upon delayed storage.Conclusion: Our study showed for the first-time distinct impact of preanalytical factors on the different forms of CSF alpha-synuclein. These findings highlight the need for special considerations for the different forms of alpha-synuclein during CSF samples’ collection and processing.

【 授权许可】

Unknown