Single-cell characterization of in vitro migration and interaction dynamics of T cells expanded with IL-2 and IL-7

Frontiers in Immunology

卷:6

Björn eÖnfelt¹  Karin eGustafsson¹  Thomas Wilhelm Frisk¹  Johanna Maria Tauriainen²  Marcus eBuggert²  Mårten eGöthlin²  Annika C Karlsson²  Michael eUhlin³  Jens eGertow³ 

[1] KTH-Royal Institute of Technology;

[2] Karolinska Institutet;

[3] Karolinska University Hospital;

关键词: Microscopy, Fluorescence;    Single-Cell Analysis;    T cell;    IL-2;    IL-7;    live-cell imaging;   

DOI  :  10.3389/fimmu.2015.00196

来源: DOAJ

【 摘 要 】

T cells are pivotal in the immune defense against cancers and infectious agents. To mount an effector response against cancer cells, T cells need to migrate to the cancer-site, engage in contacts with cancer cells and perform their effector functions. Adoptive T cell therapy is an effective strategy as treatment of complications such as relapse or opportunistic infections after hematopoietic stem cell transplantations. This requires a sufficient amount of cells that are able to expand and respond to tumor or viral antigens. The cytokines interleukin (IL)-2 and IL-7 drive T cell differentiation, proliferation and survival and are commonly used to expand T cells ex vivo. Here, we have used microchip-based live-cell imaging to follow the migration of individual T cells, their interactions with allogeneic monocytes, cell division and apoptosis for extended periods of time; something that cannot be achieved by commonly used methods. Our data indicate that cells grown in IL-7 + IL-2 had similar migration and contact dynamics as cells grown in IL-2 alone. However, the addition of IL-7 decreased cell death creating a more viable cell population, which should be beneficial when preparing cells for immunotherapy.

【 授权许可】

Unknown