Evidence of muscarinic acetylcholine receptors in the retinal centrifugal system of the chick

Brazilian Journal of Medical and Biological Research

卷:33

关键词: muscarinic receptors;    retina;    isthmo-optic nucleus;    centrifugal fibers;    acetylcholine;    chick;   

DOI  :  

来源: DOAJ

【 摘 要 】

In this study we characterize the presence of muscarinic acetylcholine receptors (mAChR) in the isthmo-optic nucleus (ION) of chicks by immunohistochemistry with the M35 antibody. Some M35-immunoreactive fibers were observed emerging from the retinal optic nerve insertion, suggesting that they could be centrifugal fibers. Indeed, intraocular injections of cholera toxin B (CTb), a retrograde tracer, and double-labeling with M35 and CTb in the ION confirmed this hypothesis. The presence of M35-immunoreactive cells and the possible mAChR expression in ION and ectopic neuron cells in the chick brain strongly suggest the existence of such a cholinergic system in this nucleus and that acetylcholine release from amacrine cells may mediate interactions between retinal cells and ION terminals.

【 授权许可】

Unknown