Evidence of muscarinic acetylcholine receptors in the retinal centrifugal system of the chick

Brazilian Journal of Medical and Biological Research

K.c. Calaza¹  P.f. Gardino¹ 

[1] ,Universidade Federal do Rio de Janeiro Instituto de Biofísica Carlos Chagas Filho Rio de Janeiro RJ ,Brasil

关键词: muscarinic receptors;    retina;    isthmo-optic nucleus;    centrifugal fibers;    acetylcholine;    chick;   

DOI  :  10.1590/S0100-879X2000000900014

来源: SciELO

【 摘 要 】

In this study we characterize the presence of muscarinic acetylcholine receptors (mAChR) in the isthmo-optic nucleus (ION) of chicks by immunohistochemistry with the M35 antibody. Some M35-immunoreactive fibers were observed emerging from the retinal optic nerve insertion, suggesting that they could be centrifugal fibers. Indeed, intraocular injections of cholera toxin B (CTb), a retrograde tracer, and double-labeling with M35 and CTb in the ION confirmed this hypothesis. The presence of M35-immunoreactive cells and the possible mAChR expression in ION and ectopic neuron cells in the chick brain strongly suggest the existence of such a cholinergic system in this nucleus and that acetylcholine release from amacrine cells may mediate interactions between retinal cells and ION terminals.

【 授权许可】

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