Evaluation of the Anticancer Potential of Crude, Irradiated Cerastes cerastes Snake Venom and Propolis Ethanolic Extract & Related Biological Alterations

Molecules

卷:26

Mohammad Y. Alfaifi¹  Serag Eldin I. Elbehairi¹  Sanaa O. Abdallah²  Mohamed A. El-Desouky²  Mostafa I. Abdelglil²  Aly F. Mohamed³ 

[1] Biology Department, Faculty of Science, King Khalid University, Abha 9004, Saudi Arabia;

[2] Faculty of Sciences, Cairo University, Giza 12613, Egypt;

[3] The International Center for Training & Advanced Researches (ICTAR–Egypt), Cairo 11647, Egypt;

关键词: PEE;    IRR V;    A549, PC3;    apoptotic;    IC₅₀;    CV;   

DOI  :  10.3390/molecules26227057

来源: DOAJ

【 摘 要 】

We aimed to evaluate the anticancer potential of crude venom (CV), γ irradiated Certastes cerastes venom (IRRV), and propolis ethanolic extract (PEE). IRRV showed a higher toxicity than CV, while CV-PEE showed higher toxicity than IRRV and CV against lung [A549] and prostate [PC3] cancer cells. Toxicity to [A549] and [PC3] cells was concentration and cell type dependent. In comparison to controls, apoptotic genes showed a significant upregulation of P53 and Casp-3 and a downregulation of Bcl-2. Also, induced elevated DNA accumulation in the [S] phase post PC3 cell treatment with IRRV and CV, as well as a significant DNA accumulation at G2/M phase after IRRV treatment of A549 cells. In contrast, PC3 cells showed a negligible cellular DNA accumulation after PEE treatment. Glutathione reductase [GR] was reduced in case of PC3 and A549 cell treated with IRRV, CV, and PEE compared with its values in untreated cell control. The Malondialdehyde [MDA] values in both cells recorded a significant elevation post IRRV treatment compared to the rest of the treatment regimen and untreated cell control. Similarly, IRRV and CV-PEE mix showed obviously higher reactive oxygen species [ROS] values than PC3 and A549 cell treatments with CV and PEE.

【 授权许可】

Unknown