In vitro evaluation of antioxidant, cytotoxic and apoptotic activities of different extracts from the leaves of Teucrium ramosissimum (Lamiaceae)

Dynamic Chiropractic

Ines Sk¹  Mohamed Ben Sghaier² 

[1] Department of Cellular and Molecular Biology, Faculty of Dental Medicine, Rue Avicenne, Monastir, 5000, Tunisia.;Department of Pharmacognosy, Faculty of Pharmacy, Rue Avicenne, Monastir, 5000, Tunisia.

关键词: Teucrium ramosissimum;    antioxidant activity;    cytotoxic;    apoptotic;    K562 cells.;   

DOI  :  10.5897/JMPR10.775

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

Thein vitroantioxidant, cytotoxic and apoptotic activities ofTeucrium ramosissimumextracts were investigated. The antioxidant activities of the tested extracts were evaluated through three chemical assays which are (1): Thecupric reducing antioxidant capacity (CUPRAC), (2) The reducing power (RP) and (3) The ferric reducing antioxidant power (FRAP).Total oligomer flavonoids enriched extract (TOF) showed the best antioxidant activity evaluated by the CUPRAC and FRAP assays withtrolox equivalent antioxidant capacity (TEAC) values of 12.85 and 0.525 µM, respectively compared to control. Whereasethyl acetate (EA) extractexhibited the highest reducing power when using the RP assay with a TEAC value of 2.025 µM. Furthermore, the effects ofT. ramosissimumextractson cell proliferation and induction ofdeoxyribonucleic acid(DNA) degradation, as an apoptotic marker in human leukemia cells were also examined. The cytotoxic activity revealed that themethanol extractinhibited significantly the proliferation of K562 cells (IC50=150 µg/ml), however no specific DNA degradation indicating an apoptotic effect was observed.

【 授权许可】

Unknown