Exercise-Induced Browning of White Adipose Tissue and Improving Skeletal Muscle Insulin Sensitivity in Obese/Non-obese Growing Mice: Do Not Neglect Exosomal miR-27a

Frontiers in Nutrition

卷:9

Lihong Jia²  Lingling Zhai²  Yinglong Bai²  Wei Wei²  Hongkun Jiang³  Li Zhang⁴  Yibai Li⁵  Dongxue Wang⁶  Xihuan Zhang⁷ 

[1] Department of Dermatology, First Hospital of China Medical University, Shenyang, China;

[2] Department of Maternal and Child Health, School of Public Health, China Medical University, Shenyang, China;

[3] Department of Pediatrics, First Hospital of China Medical University, Shenyang, China;

[4] Key Laboratory of Immunodermatology, Ministry of Education and NHC, National Joint Engineering Research Center for Theranostics of Immunological Skin Diseases, Shenyang, China;

[5] The First Division of Clinical Medicine, China Medical University, Shenyang, China;

[6] The Second People’s Hospital of Jiashan, Jiaxing, China;

[7] Xinzhou District Center for Disease Control and Prevention, Wuhan, China;

关键词: exercise;    exosomes;    miR-27a;    browning effect of white adipose tissue;    skeletal muscle;    insulin sensitivity;   

DOI  :  10.3389/fnut.2022.940673

来源: DOAJ

【 摘 要 】

Exercise is considered as a favorable measure to prevent and treat childhood obesity. However, the underlying mechanisms of exercise-induced beneficial effects and the difference between obese and non-obese individuals are largely unclear. Recently, miR-27a is recognized as a central upstream regulator of proliferator-activated receptor γ (PPAR-γ) in contributing to various physiological and pathological processes. This study aims to explore the possible cause of exercise affecting white adipose tissue (WAT) browning and reversing skeletal muscle insulin resistance in obese/non-obese immature bodies. For simulating the process of childhood obesity, juvenile mice were fed with a basal diet or high-fat diet (HFD) and took 1 or 2 h swimming exercise simultaneously for 10 weeks. The obese animal model was induced by the HFD. We found that exercise hindered HFD-induced body fat development in growing mice. Exercise modified glucolipid metabolism parameters differently in the obese/non-obese groups, and the changes of the 2 h exercise mice were not consistent with the 1 h exercise mice. The level of serum exosomal miR-27a in the non-exercise obese group was increased obviously, which was reduced in the exercise obese groups. Results from bioinformatics analysis and dual-luciferase reporter assay showed that miR-27a targeted PPAR-γ. Exercise stimulated WAT browning; however, the response of obese WAT lagged behind normal WAT. In the HFD-fed mice, 2 h exercise activated the IRS-1/Akt/GLUT-4 signaling pathway in the skeletal muscles. In summary, our findings confirmed that exercise-induced beneficial effects are associated with exercise duration, and the response of obese and non-obese bodies is different. Exosomal miR-27a might be a crucial node for the process of exercise-induced browning of WAT and improving skeletal muscle insulin sensitivity.

【 授权许可】

Unknown