Proteomic Insights into Sulfur Metabolism inthe Hydrogen-Producing HyperthermophilicArchaeon Thermococcus onnurineus NA1

International Journal of Molecular Sciences

卷:16

Sung-Ho Yun¹  Soo Jung Kim¹  Joseph Kwon¹  Seung Il Kim¹  Young-Ho Chung¹  Yoon-Jung Moon¹  Jonghyun Kim¹  Hye Li Lim¹  Sung Gyun Kang²  Jung-Hyun Lee² 

[1] Division of Life Science, Korea Basic Science Institute, Daejeon 305-806, Korea;

[2] Marine Biotechnology Research Division, Korea Institute of Ocean Science and Technology,Ansan 426-744, Korea;

关键词: Thermococcus onnurineus NA1;    nano-UPLC-MSE;    comparative proteomics;    elemental sulfur;    H2S;    hydrogenases;    sulfur metabolism;    oxidative stress defense;   

DOI  :  10.3390/ijms16059167

来源: DOAJ

【 摘 要 】

The hyperthermophilic archaeon Thermococcus onnurineus NA1 has been shown to produce H2 when using CO, formate, or starch as a growth substrate. This strain can also utilize elemental sulfur as a terminal electron acceptor for heterotrophic growth. To gain insight into sulfur metabolism, the proteome of T. onnurineus NA1 cells grown under sulfur culture conditions was quantified and compared with those grown under H2-evolving substrate culture conditions. Using label-free nano-UPLC-MSE-based comparative proteomic analysis, approximately 38.4% of the total identified proteome (589 proteins) was found to be significantly up-regulated (≥1.5-fold) under sulfur culture conditions. Many of these proteins were functionally associated with carbon fixation, Fe–S cluster biogenesis, ATP synthesis, sulfur reduction, protein glycosylation, protein translocation, and formate oxidation. Based on the abundances of the identified proteins in this and other genomic studies, the pathways associated with reductive sulfur metabolism, H2-metabolism, and oxidative stress defense were proposed. The results also revealed markedly lower expression levels of enzymes involved in the sulfur assimilation pathway, as well as cysteine desulfurase, under sulfur culture condition. The present results provide the first global atlas of proteome changes triggered by sulfur, and may facilitate an understanding of how hyperthermophilic archaea adapt to sulfur-rich, extreme environments.

【 授权许可】

Unknown