Proteomic Insights into Sulfur Metabolism in the Hydrogen-Producing Hyperthermophilic Archaeon Thermococcus onnurineus NA1

International Journal of Molecular Sciences

Yoon-Jung Moon²  Joseph Kwon²  Sung-Ho Yun²  Hye Li Lim²  Jonghyun Kim²  Soo Jung Kim²  Sung Gyun Kang¹  Jung-Hyun Lee¹  Seung Il Kim²  Young-Ho Chung² 

[1] Marine Biotechnology Research Division, Korea Institute of Ocean Science and Technology, Ansan 426-744, Korea; E-Mails:;Division of Life Science, Korea Basic Science Institute, Daejeon 305-806, Korea; E-Mails:

关键词: Thermococcus onnurineus NA1;    nano-UPLC-MS^E;    comparative proteomics;    elemental sulfur;    H₂S;    hydrogenases;    sulfur metabolism;    oxidative stress defense;   

DOI  :  10.3390/ijms16059167

来源: mdpi

【 摘 要 】

The hyperthermophilic archaeon Thermococcus onnurineus NA1 has been shown to produce H₂ when using CO, formate, or starch as a growth substrate. This strain can also utilize elemental sulfur as a terminal electron acceptor for heterotrophic growth. To gain insight into sulfur metabolism, the proteome of T. onnurineus NA1 cells grown under sulfur culture conditions was quantified and compared with those grown under H₂-evolving substrate culture conditions. Using label-free nano-UPLC-MS^E-based comparative proteomic analysis, approximately 38.4% of the total identified proteome (589 proteins) was found to be significantly up-regulated (≥1.5-fold) under sulfur culture conditions. Many of these proteins were functionally associated with carbon fixation, Fe–S cluster biogenesis, ATP synthesis, sulfur reduction, protein glycosylation, protein translocation, and formate oxidation. Based on the abundances of the identified proteins in this and other genomic studies, the pathways associated with reductive sulfur metabolism, H₂-metabolism, and oxidative stress defense were proposed. The results also revealed markedly lower expression levels of enzymes involved in the sulfur assimilation pathway, as well as cysteine desulfurase, under sulfur culture condition. The present results provide the first global atlas of proteome changes triggered by sulfur, and may facilitate an understanding of how hyperthermophilic archaea adapt to sulfur-rich, extreme environments.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.