Purification, characterization and antioxidant activities in vitro of polysaccharides from Amaranthus hybridus L.

PeerJ

卷:8

Caixia Zhou¹  Wenjun Sun¹  Xiaoli Wang¹  Yi Cai¹  Hui Chen¹  Huipeng Yao¹  Zhi Shan¹  Tongliang Bu¹  Yujia Tang¹  Zizhong Tang¹  Tianrun Zheng¹  Lin Gou¹  Yirong Xiao²  Lin Huang³ 

[1] College of Life Sciences, Sichuan Agricultural University, Yaan, China;

[2] Sichuan Agricultural University Hospital, Yaan, China;

[3] Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China;

关键词: Amaranthus hybridus L.;    Physicochemical;    Polysaccharides;    Antioxidant;   

DOI  :  10.7717/peerj.9077

来源: DOAJ

【 摘 要 】

Background Amaranthus hybridus L. is an annual, erect or less commonly ascending herb that is a member of the Amaranthaceae family. Polysaccharides extracted from traditional Chinese medicines may be effective substances with antioxidant activity. Methods In this study, we isolated crude polysaccharides from A. hybridus (AHP-M) using microwave-assisted extraction. Then, the AHP-M was purified by chromatography with DEAE-32 cellulose, and two fractions, AHP-M-1 and AHP-M-2, were obtained. The structural characteristics of AHP-M-1 and AHP-M-2 were investigated, and their antioxidant activities were analyzed in vitro. Results We found that the monosaccharide composition of AHP-M-1 was different from that of AHP-M-2. The molecular weights of AHP-M-1 and AHP-M-2 were 77.625 kDa and 93.325 kDa, respectively. The results showed that the antioxidant activity of AHP-M-2 was better than that of AHP-M-1. For AHP-M-2, the DPPH radical scavenging rate at a concentration of 2 mg/mL was 78.87%, the hydroxyl radical scavenging rate was 39.34%, the superoxide anion radical scavenging rate was 80.2%, and the reduction ability of Fe3+ was approximately 0.90. The total antioxidant capacity per milligram of AHP-M-2 was 6.42, which was higher than that of Vitamin C (Vc). Conclusion The in vitro test indicated that AHP-M-1 and AHP-M-2 have good antioxidant activity, demonstrating that A. hybridus L. polysaccharide has immense potential as a natural antioxidants.

【 授权许可】

Unknown