Physicochemical and antioxidant properties of Bangladeshi honeys stored for more than one year

BMC Complementary and Alternative Medicine

Research Article

Siti Amrah Sulaiman¹  Mohammed Moniruzzaman¹  Ibrahim Khalil²  Nazmul Islam²  Siew Hua Gan³  Asiful Islam³  Abdul Mottalib⁴ 

[1] Department of Pharmacology, School of Medical Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia;Department of Pharmacology, School of Medical Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia;Department of Biochemistry and Molecular Biology, Jahangirnagar University, 1342, Savar, Dhaka, Bangladesh;Human Genome Centre, School of Medical Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia;Laboratory Services Division, Bangladesh Institute of Research and Rehabilitation in Diabetes, Endocrine and Metabolic Disorders Hospital (BIRDEM), Shahbag, Dhaka, Bangladesh;

关键词: Bangladeshi honey;    Antioxidant;    Physicochemical;    Phenolics;    Proline;   

DOI  :  10.1186/1472-6882-12-177

 received in 2012-07-09, accepted in 2012-10-05,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundThere is no available information on physicochemical and antioxidant properties on Bangladeshi honey. We investigated five different monofloral and three different multifloral honey samples collected from different parts of Bangladesh.MethodsThe levels of phenolics, flavonoids, ascorbic acid, ascorbic acid equivalent antioxidant content (AEAC), proline, protein and antioxidants were determined in the honey samples using ferric reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays.ResultsThe highest level of phenolic was 688.5 ± 5.9 mg Gallic acid/kg, and the highest level of flavonoid was 155 ± 6.9 mg Catechin/kg. The highest color intensity was 2034.00 ± 17.5 mAU, and the highest protein content was 8.6 ± 0.0mg/g. High levels of proline (2932.8 ± 3.7 mg/kg), ascorbic acid (154.3 ± 0.3 mg/kg), AEAC (34.1 ± 1.4mg/100 g) and FRAP (772.4 ± 2.5 μmol Fe (II)/100 g) were detected in some of the samples, especially the multifloral honey samples, indicating good antioxidant properties. A strong positive correlation was found between phenolics, flavonoids, DPPH, FRAP and color intensity, indicating that in addition to total phenolic and flavonoid concentrations, color intensity and amino acid are good indicators of the antioxidant potential of honey. Except for a single sample (BDH-6), the honey samples stored for 1.5 years at room temperature still had 5-hydroxymethylfurfural (HMF) values within the recommended range (mean = 10.93 mg/kg), indicating that the rate of HMF production in Bangladeshi honey samples is low.ConclusionIt is postulated that the low rate of HMF formation could be attributed to the acidic and low moisture content in the samples. In general, multifloral honeys have higher antioxidant properties based on their high levels of phenolics, flavonoids, AEAC, DPPH and FRAP when compared to monofloral honeys. We also found that monofloral honey samples from Guizotia abyssinica and Nigella sativa had high antioxidant properties.

【 授权许可】

Unknown   
© Islam et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.