Relationship between Sperm Parameters and DNA Fragmentation using a Halosperm Kit

Anatomical Sciences Journal

卷:10

Mir Mehrdad Farsi¹  Soraya Khafri¹  Houshang Rafighdoost¹  Maryam Javadi¹ 

[1] ;

关键词: spermatozoa;    infertility;    dna fragmentation;   

DOI  :  

来源: DOAJ

【 摘 要 】

Introduction: tegrity is the most important component for transferring genetic information Sperm DNA fragmentationis considered a component of male infertility. Its assessment comprises conventional semen analysis of whichemphasis is placed on its evaluation in fertility clinics. In this study, we investigate the relationship between spermparameters of motility, morphology, concentration, and DNA fragmentation level (DFLs) in sperm.Materials and Methods: 89: nfertile men were assessed by conventional sperm analysis. DFLs were determined byHalosperm, a sperm chromatin dispersion test (SCD) which was performed in both groups of patients, the low DNAfragmentation group (LFG≤30%) and high DNA fragmentation group (HFG) 30%.Results: Sperm parameters of concentration (p<0.001), motility (p<0.001) and morphology (p<0.01) in the HFG groupwere significantly lower than in the LF group after sperm preparation. Logistic regression model showed morphologyand motility variables were predictive of DFLs. The cut off points were 5% (morphology) and 50% (motility).Conclusion: Our results have shown a negative relationship between DFLs and sperm parameters after preparation.According to a DF value of >30% in the percent of men with normal semen parameters, the high importance ofperforming a DF test in the clinic setting in order to determine sperm DNA problems and the presence of spermabnormalities in patients is recommended.

【 授权许可】

Unknown