Cartilage Formation In Vivo Using High Concentration Collagen-Based Bioink with MSC and Decellularized ECM Granules

International Journal of Molecular Sciences

卷:23

Evgeny E. Beketov¹  Anastas A. Kisel¹  Vasiliy M. Petriev¹  Grigory A. Demyashkin¹  Egor P. Malakhov¹  Tatiana S. Lagoda¹  Elena V. Isaeva¹  Anna N. Smirnova¹  Nina D. Yakovleva¹  Nadezhda V. Arguchinskaya¹  Sergey A. Ivanov¹  Sergey P. Domogatsky²  Egor O. Osidak²  Petr S. Eremin³  Petr V. Shegay⁴  Andrey D. Kaprin⁴ 

[1] A Tsyb Medical Radiological Research Centre, Branch of the National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation, 249036 Obninsk, Russia;

[2] Imtek Ltd., 121552 Moscow, Russia;

[3] National Medical Research Center of Rehabilitation and Balneology of Ministry of Healthcare of the Russian Federation, Federal State Budgetary Institution, 121099 Moscow, Russia;

[4] National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation, 249036 Obninsk, Russia;

关键词: bioprinting;    collagen;    bioink;    MSC;    ECM;    cartilage;   

DOI  :  10.3390/ijms23052703

来源: DOAJ

【 摘 要 】

The aim of this study was to verify the applicability of high-concentration collagen-based bioink with MSC (ADSC) and decellularized ECM granules for the formation of cartilage tissue de novo after subcutaneous implantation of the scaffolds in rats. The printability of the bioink (4% collagen, 2.5% decellularized ECM granules, derived via 280 μm sieve) was shown. Three collagen-based compositions were studied: (1) with ECM; (2) with MSC; (3) with ECM and MSC. It has been established that decellularized ECM granules are able to stimulate chondrogenesis both in cell-free and MSC-laden scaffolds. Undesirable effects have been identified: bone formation as well as cartilage formation outside of the scaffold area. The key perspectives and limitations of ECM granules (powder) application have been discussed.

【 授权许可】

Unknown