Exploring metal availability in the natural niche of Streptococcus pneumoniae to discover potential vaccine antigens

Virulence

卷:11

Joen Luirink¹  Wouter S. P. Jong¹  Diane Houben¹  H. Bart van den Berg van Saparoea¹  Marien I. de Jonge²  Lucille F. van Beek²  Fred van Opzeeland²  Irma Joosten²  Dimitri A. Diavatopoulos²  Christa E. van der Gaast – de Jongh²  Thomas H. A. Ederveen³  Daniela M. Ferreira⁴  Elena Mitsi⁴  Kristin Surmann⁵  Christian Hentschker⁵  Uwe Völker⁵  Frank Schmidt⁵ 

[1] Abera Bioscience AB;

[2] Radboud Institute for Molecular Life Sciences;

[3] Radboud University Medical Center;

[4] Respiratory Infection Group;

[5] University Medicine Greifswald;

关键词: streptococcus pneumoniae;    transition metals;    nasal fluid;    protein antigens;    colonization;    in vivo-mimicking;   

DOI  :  10.1080/21505594.2020.1825908

来源: DOAJ

【 摘 要 】

Nasopharyngeal colonization by Streptococcus pneumoniae is a prerequisite for pneumococcal transmission and disease. Current vaccines protect only against disease and colonization caused by a limited number of serotypes, consequently allowing serotype replacement and transmission. Therefore, the development of a broadly protective vaccine against colonization, transmission and disease is desired but requires a better understanding of pneumococcal adaptation to its natural niche. Hence, we measured the levels of free and protein-bound transition metals in human nasal fluid, to determine the effect of metal concentrations on the growth and proteome of S. pneumoniae. Pneumococci cultured in medium containing metal levels comparable to nasal fluid showed a highly distinct proteomic profile compared to standard culture conditions, including the increased abundance of nine conserved, putative surface-exposed proteins. AliA, an oligopeptide binding protein, was identified as the strongest protective antigen, demonstrated by the significantly reduced bacterial load in a murine colonization and a lethal mouse pneumonia model, highlighting its potential as vaccine antigen.

【 授权许可】

Unknown