Ultrasmall Superparamagnetic Iron Oxide Labeled Silk Fibroin/Hydroxyapatite Multifunctional Scaffold Loaded With Bone Marrow-Derived Mesenchymal Stem Cells for Bone Regeneration

Frontiers in Bioengineering and Biotechnology

卷:8

Zelong Chen¹  Chenggong Yan¹  Yikai Xu¹  Qin Liu¹  Dan Li²  Yong Lan²  Yu Liu²  Longbao Feng³ 

[1] Department of Medical Imaging Center, Nanfang Hospital, Southern Medical University, Guangzhou, China;

[2] Guangzhou Beogene Biotech Co., Ltd., Guangzhou, China;

[3] Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Guangdong Provincial Engineering and Technological Research Center for Drug Carrier Development, Department of Biomedical Engineering, Jinan University, Guangzhou, China;

关键词: tissue engineering;    multifunctional scaffold;    bone marrow-derived mesenchymal stem cells;    bone regeneration;    magnetic resonance imaging;   

DOI  :  10.3389/fbioe.2020.00697

来源: DOAJ

【 摘 要 】

Numerous tissue-engineered constructs have been investigated as bone scaffolds in regenerative medicine. However, it remains challenging to non-invasively monitor the biodegradation and remodeling of bone grafts after implantation. Herein, silk fibroin/hydroxyapatite scaffolds incorporated with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles were successfully synthesized, characterized, and implanted subcutaneously into the back of nude mice. The USPIO labeled scaffolds showed good three-dimensional porous structures and mechanical property, thermal stability for bone repair. After loaded with bone marrow-derived mesenchymal stem cells (BMSCs), the multifunctional scaffolds promoted cell adhesion and growth, and facilitated osteogenesis by showing increased levels of alkaline phosphatase activity and up-regulation of osteoblastic genes. Furthermore, in vivo quantitative magnetic resonance imaging (MRI) results provided valuable information on scaffolds degradation and bone formation simultaneously, which was further confirmed by computed tomography and histological examination. These findings demonstrated that the incorporation of USPIO into BMSCs-loaded multifunctional scaffold system could be feasible to noninvasively monitor bone regeneration by quantitative MRI. This tissue engineering strategy provides a promising tool for translational application of bone defect repair in clinical scenarios.

【 授权许可】

Unknown