Effects of Pomegranate (Punica Granatum L.) Seed and Peel Methanolic Extracts on OxidativeStress and Lipid Profile Changes Induced by Methotrexate in Rats

Advanced Pharmaceutical Bulletin

卷:7

Roxana Vafafar¹  Rogayeh Amini Afshar²  Aliasghar Pouri³  Parvin Zakeri-Milani³  Farideh Doostan⁴  Mehran Mesgari Abbasi⁵ 

[1] Department of Biology, Faculty of Science, Islamic Azad University, Ahar Branch, Ahar, Iran. ;

[2] Faculty of Sciences, Urmia University, Urmia, Iran.;

[3] Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.;

[4] Physiology Research Center, Kerman University of Medical Sciences, Kerman, Iran. ;

[5] Student Research Committee, Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.;

关键词: Methotrexate;    Oxidative stress;    Pomegranate;    Rats;   

DOI  :  10.15171/apb.2017.032

来源: DOAJ

【 摘 要 】

Purpose: Methotrexate(MTX) is prescribed in many diseases and can result in oxidative stress (OS)followed by injuries in some tissues. Antioxidants administration are effectivein reducing OS. Pomegranate exhibits high anti-oxidant capacities. This studyinvestigated whether pomegranate seed and peel methanolic extracts (PSE andPPE) could protect against MTX-induced OS and lipid profile changes in rats.Methods: Forty-eight rats were randomlydivided into 6 groups: control group (normal salin), PSE group (500 mg/kg,orally), PPE group (500 mg/kg, orally), MTX group (10 mg/kg, IM), MTX and PSE group, and MTX and PPE group. Blood samples weretaken for analysis in the end of the procedure.Results:The findings showed a significant reduction in Glutathione peroxidase (GPx) andSuperoxide dismutase (SOD), and an enhancement in malondialdehyde (MDA) valuesafter MTX treatment (p < 0.05). SOD and GPx levels reached the levels of thecontrol group in MTX+SPE and MTX+PPE groups. No significant differences wereobserved in catalase (CAT) and total antioxidant capacity (TAC) levels betweengroups. The results showed a significant decrease in total cholesterol (TC),low density lipoprotein (LDL), and high density lipoprotein (HDL) in the MTXtreated group (p < 0.01). The values of TC, HDL, and LDL became elevated tothe normal control levels in the MTX+PSE and MTX+PPE treated groups.Conclusion: The results showed the OS induced by MTX and theprotective effects of PSE and PPE against MTX-induced serum oxidative stressand lipid profile changes in rats.

【 授权许可】

Unknown