Salivary β-glucosidase as a direct factor influencing the occurrence of halitosis

Biochemistry and Biophysics Reports

卷:26

Lucimari Teixeira Essenfelder¹  Maria de Lourdes Borba Magalhães¹  Anderson Albino Gomes¹  Gustavo Felippe da Silva¹  Marcelo Alves Moreira²  David José Miquelluti²  Jefferson Luis Meirelles Coimbra²  Sandra Maria Ferraz³ 

[1] Biochemistry Laboratory, Center of Agroveterinary Sciences, State University of Santa Catarina, Lages, Santa Catarina, 88520-000, Brazil;

[2] Department of Soil and Natural Resources, Center of Agroveterinary Sciences, State University of Santa Catarina, Lages, Santa Catarina, 88520-000, Brazil;

[3] Department of Veterinary Medicine, Center of Agroveterinary Sciences, State University of Santa Catarina, Lages, Santa Catarina, 88520-000, Brazil;

关键词: Biofilm;    Halitosis;    Oral health;    Saliva;    β-Glucosidase;   

DOI  :  

来源: DOAJ

【 摘 要 】

β-Glucosidases are enzymes present in all living organisms, playing a pivotal role in diverse biological processes. These enzymes cleave β-glycosidic bonds between carbohydrates, or between a carbohydrate and a non-carbohydrate moiety, which may result in the liberation of volatile aglycones. Released compounds execute diverse physiological roles, while the industry takes advantage of exogenously added β-glucosidases for aroma enrichment during food and beverage production. β-Glucosidase enzymatic activity has been reported in human saliva and given the fact that these enzymes are involved in aroma release, we investigated here the correlation between β-glucosidase activity in human saliva and the occurrence of halitosis. Measurement of salivary enzyme activity of 48 volunteers was performed using p-nitrophenyl-β-d-glucopyranoside as substrate. Each volunteer was clinically evaluated by a dental surgeon and clinical and laboratorial data were statistically analyzed. Gas-chromatography of saliva headspace allowed the analysis of the direct role of exogenous β-glucosidase on aromatic /volatile profile of saliva samples. The data demonstrated a positive correlation between halitosis and enzymatic activity, suggesting that the enzyme exerts a direct role in the occurrence of bad breath. Gas-chromatography analysis demonstrated that exogenously added enzyme led to the alteration of volatile organic content, confirming a direct contribution of β-glucosidase activity on saliva volatile compounds release. Although halitosis is a multifactorial condition, the complete understanding of all governing factors may allow the development of more effective treatment strategies. Such studies may pave the way to the use of β-glucosidase inhibitors for halitosis clinical management.

【 授权许可】

Unknown